Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family.
Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family.
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DOI：
10.1371/journal.pone.0128025
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Bhave M
中科院分区：
文献类型：
--
作者：
Hove RM;Ziemann M;Bhave M
水通道蛋白(Aquaporins，AQP)是调节水分和其他底物跨细胞膜双向流动的主要内源蛋白，在植物-水分关系、干旱胁迫反应和作物生产力中发挥着关键作用。然而，到目前为止，关于这些蛋白质对主要农作物大麦(大麦)的生理贡献的数据有限。本文报道了大麦MIP家族的鉴定和表达分析。对公开可用的叶mRNA-seq数据、大麦基因组草稿数据、GenBank转录本和16个新注释的全面搜索表明，大麦MIP家族至少由40个AQP组成。在两个质膜内源蛋白(PIP)AQP中可能存在选择性剪接事件。对AQP信号序列和特异性决定位置的分析表明，几种可能的AQP亚型可能运输包括生理重要底物在内的非水底物，并对非生物胁迫做出反应。对我们公开的叶片mRNA-seq数据的分析发现，HvPIP1；2和HvTIP4；1在盐胁迫下有显著的差异表达。对其他基因表达资源的分析也证实了不同组织和/或对盐度的响应的同工型特异性反应，以及一些潜在的品种间差异。这项工作系统和全面地分析了大麦AQP基因的大部分(如果不是全部)、它们的序列、在不同组织中的表达模式、潜在的运输和胁迫响应功能，以及为选择和/或培育耐逆大麦品种提供了强有力的框架。此外，这些大麦数据将对进化关系密切的小麦(Triticum aestivum L.)的遗传研究具有很高的价值。
Aquaporins (AQPs) are major intrinsic proteins (MIPs) that mediate bidirectional flux of water and other substrates across cell membranes, and play critical roles in plant-water relations, dehydration stress responses and crop productivity. However, limited data are available as yet on the contributions of these proteins to the physiology of the major crop barley (Hordeum vulgare). The present work reports the identification and expression analysis of the barley MIP family. A comprehensive search of publicly available leaf mRNA-seq data, draft barley genome data, GenBank transcripts and sixteen new annotations together revealed that the barley MIP family is comprised of at least forty AQPs. Alternative splicing events were likely in two plasma membrane intrinsic protein (PIP) AQPs. Analyses of the AQP signature sequences and specificity determining positions indicated a potential of several putative AQP isoforms to transport non-aqua substrates including physiological important substrates, and respond to abiotic stresses. Analysis of our publicly available leaf mRNA-seq data identified notable differential expression of HvPIP1;2 and HvTIP4;1 under salt stress. Analyses of other gene expression resources also confirmed isoform-specific responses in different tissues and/or in response to salinity, as well as some potentially inter-cultivar differences. The work reports systematic and comprehensive analysis of most, if not all, barley AQP genes, their sequences, expression patterns in different tissues, potential transport and stress response functions, and a strong framework for selection and/or development of stress tolerant barley varieties. In addition, the barley data would be highly valuable for genetic studies of the evolutionarily closely related wheat (Triticum aestivum L.).
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