A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles
A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles
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DOI：
10.1021/ja034775u
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发表时间：
2003-06-04
影响因子：
15
通讯作者：
Lu, Y
中科院分区：
文献类型：
--
作者：
Liu, JW;Lu, Y
报道了一种基于DNA酶介导的纳米金组装的高灵敏度和高选择性的铅比色生物传感器。它由DNA酶及其底物组成，该底物可以与附着在金纳米颗粒上的5 '-硫代修饰的DNA杂交。杂交将金纳米颗粒聚集在一起，产生蓝色的纳米颗粒组装体。在铅的存在下，DNA酶催化特定的水解裂解，这阻止了纳米颗粒组装体的形成，导致红色的单个纳米颗粒。通过添加DNAzyme的非活性变体，检测水平可以调节到几个数量级，从100 nM到超过200 μM。这里开发的概念可以应用于核酸酶/纳米颗粒传感器的设计，用于体外选择的分析物，从而可以显着扩大纳米材料的应用范围，并提供一种新的方法来设计简单的比色生物传感器。
A highly sensitive and selective colorimetric lead biosensor based on DNAzyme-directed assembly of gold nanoparticles is reported. It consists of a DNAzyme and its substrate that can hybridize to a 5‘-thio-modified DNA attached to gold nanoparticles. The hybridization brings gold nanoparticles together, resulting in a blue-colored nanoparticle assembly. In the presence of lead, the DNAzyme catalyzes specific hydrolytic cleavage, which prevents the formation of the nanoparticle assembly, resulting in red-colored individual nanoparticles. The detection level can be tuned to several orders of magnitude, from 100 nM to over 200 μM, through addition of an inactive variant of the DNAzyme. The concept developed here can be applied to the design of nucleic acid enzyme/nanoparticle sensors for analytes that are subject to in vitro selection, and thus can significantly expand the scope of nanomaterial applications and provide a novel approach to designing simple colorimetric biosensors.