Replication and shedding of MERS-CoV in Jamaican fruit bats (Artibeus jamaicensis).
Replication and shedding of MERS-CoV in Jamaican fruit bats (Artibeus jamaicensis).
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DOI：
10.1038/srep21878
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发表时间：
2016-02-22
影响因子：
4.6
通讯作者：
Bowen RA
中科院分区：
文献类型：
--
作者：
Munster VJ;Adney DR;van Doremalen N;Brown VR;Miazgowicz KL;Milne-Price S;Bushmaker T;Rosenke R;Scott D;Hawkinson A;de Wit E;Schountz T;Bowen RA
中东呼吸综合征冠状病毒（MERS-CoV）的出现凸显了乙型冠状病毒的人畜共患潜力。对中东呼吸综合征冠状病毒起源的调查主要集中在两个潜在宿主：蝙蝠和骆驼。在这里，我们研究了蝙蝠作为中东呼吸综合征冠状病毒潜在宿主的作用。在体外，MERS-CoV刺突糖蛋白与牙买加果蝠（Artibeus jamaicensis）二肽基肽酶4 （DPP4）受体相互作用，MERS-CoV在牙买加果蝠细胞中高效复制，表明MERS-CoV在受体或细胞水平上不受限制。为了阐明宿主-病毒的内在关系，我们用MERS-CoV接种了10只牙买加果蝠。尽管所有蝙蝠都显示出感染的迹象，但没有一只蝙蝠显示出疾病的临床症状。在呼吸道和肠道中检测到病毒脱落长达9天。中东呼吸综合征冠状病毒在呼吸道短暂复制，在较小程度上在肠道和内脏复制；仅在肺部观察到有限的组织病理学改变。对肺中先天基因表达的分析显示了适度的、短暂的表达诱导。我们的研究结果表明，MERS-CoV在没有疾病临床症状的蝙蝠中保持复制能力，支持了蝙蝠是MERS-CoV祖先储存库的一般假设。
The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the zoonotic potential of Betacoronaviruses. Investigations into the origin of MERS-CoV have focused on two potential reservoirs: bats and camels. Here, we investigated the role of bats as a potential reservoir for MERS-CoV. In vitro, the MERS-CoV spike glycoprotein interacted with Jamaican fruit bat (Artibeus jamaicensis) dipeptidyl peptidase 4 (DPP4) receptor and MERS-CoV replicated efficiently in Jamaican fruit bat cells, suggesting there is no restriction at the receptor or cellular level for MERS-CoV. To shed light on the intrinsic host-virus relationship, we inoculated 10 Jamaican fruit bats with MERS-CoV. Although all bats showed evidence of infection, none of the bats showed clinical signs of disease. Virus shedding was detected in the respiratory and intestinal tract for up to 9 days. MERS-CoV replicated transiently in the respiratory and, to a lesser extent, the intestinal tracts and internal organs; with limited histopathological changes observed only in the lungs. Analysis of the innate gene expression in the lungs showed a moderate, transient induction of expression. Our results indicate that MERS-CoV maintains the ability to replicate in bats without clinical signs of disease, supporting the general hypothesis of bats as ancestral reservoirs for MERS-CoV.