RELATIONSHIP BETWEEN LEGIONELLA-PNEUMOPHILA AND ACANTHAMOEBA-POLYPHAGA - PHYSIOLOGICAL STATUS AND SUSCEPTIBILITY TO CHEMICAL INACTIVATION
RELATIONSHIP BETWEEN LEGIONELLA-PNEUMOPHILA AND ACANTHAMOEBA-POLYPHAGA - PHYSIOLOGICAL STATUS AND SUSCEPTIBILITY TO CHEMICAL INACTIVATION
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DOI：
10.1128/aem.58.8.2420-2425.1992
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发表时间：
1992-08-01
影响因子：
4.4
通讯作者：
GILBERT, P
中科院分区：
文献类型：
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作者：
BARKER, J;BROWN, MRW;GILBERT, P
对嗜肺军团菌细胞进行了存活研究，这些细胞在多食阿米巴中细胞内生长，然后暴露于聚双胍（PHMB）、苯并异噻唑酮（BIT）和5-氯-N-甲基异噻唑酮（CMIT）。同时测定了L.嗜肺菌在铁充足和铁缺乏条件下生长。BIT对铁耗竭下生长的细胞相对无效;相反，铁耗竭条件增加了细胞对PHMB和CMIT的亲合性。三种杀菌剂对L.在变形虫中生长的嗜肺菌。PHMB（1x MIC）在培养液中生长6 h内，存活率降低99.99%，24 h时未检测到存活细胞，但在阿米巴中生长的细胞在6 h和24 h时分别仅杀灭90%和99.9%。研究了3种杀菌剂对A.多食性昆虫也被确定。大多数阿米巴原虫从BIT治疗中恢复，但很少（如果有的话）存活于CMIT治疗或暴露于PHMB。这项研究不仅显示了阿米巴内生长对乳酸杆菌生理状态和抗菌药物敏感性的深刻影响。pneumophila，但也揭示PHMB是有效水处理的潜在杀生物剂。在这方面，PHMB具有显著的活性，低于其推荐使用浓度，对宿主阿米巴和L。嗜肺菌
Survival studies were conducted on Legionella pneumophila cells that had been grown intracellularly in Acanthamoeba polyphaga and then exposed to polyhexamethylene biguanide (PHMB), benzisothiazolone (BIT), and 5-chloro-N-methylisothiazolone (CMIT). Susceptibilities were also determined for L. pneumophila grown under iron-sufficient and iron-depleted conditions. BIT was relatively ineffective against cells grown under iron depletion; in contrast, iron-depleted conditions increased the susceptibilities of cells to PHMB and CMIT. The activities of all three biocides were greatly reduced against L. pneumophila grown in amoebae. PHMB (1x MIC) gave 99.99% reductions in viability for cultures grown in broth within 6 h and no detectable survivors at 24 h but only 90 and 99.9% killing at 6 h and 24 h, respectively, for cells grown in amoebae. The antimicrobial properties of the three biocides against A. polyphaga were also determined. The majority of amoebae recovered from BIT treatment, but few, if any, survived CMIT treatment or exposure to PHMB. This study not only shows the profound effect that intra-amoebal growth has on the physiological status and antimicrobial susceptibility of L. pneumophila but also reveals PHMB to be a potential biocide for effective water treatment. In this respect, PHMB has significant activity, below its recommended use concentrations, against both the host amoeba and L. pneumophila.