SHORT-TERM GLUCOCORTICOID MANIPULATIONS AFFECT NEURONAL MORPHOLOGY AND SURVIVAL IN THE ADULT DENTATE GYRUS
SHORT-TERM GLUCOCORTICOID MANIPULATIONS AFFECT NEURONAL MORPHOLOGY AND SURVIVAL IN THE ADULT DENTATE GYRUS
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DOI：
10.1016/0306-4522(90)90407-u
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发表时间：
1990-01-01
期刊：
影响因子：
3.3
通讯作者：
MCEWEN, BS
中科院分区：
文献类型：
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作者：
GOULD, E;WOOLLEY, CS;MCEWEN, BS
为了确定短期糖皮质激素操作是否会影响成年哺乳动物海马结构中神经元的形态和存活，我们对假手术雄性大鼠、肾上腺切除雄性大鼠和接受皮质酮替代的肾上腺切除雄性大鼠脑中的Golgi浸渍和Nissl染色组织进行了定量分析。肾上腺切除术后3天，在齿状回颗粒细胞层中观察到大量细胞死亡，如通过固缩细胞数量的急剧增加所检测到的。肾上腺切除术后7天，固缩细胞的数量甚至更大。此外，在肾上腺切除术后7天，高尔基体浸渍的齿状回颗粒细胞的横截细胞体面积和树突分支点的数量显着减少。皮质酮替代肾上腺切除大鼠防止出现大量的固缩细胞，以及颗粒细胞横截细胞体面积和树突状分支点的数量减少。相反，肾上腺切除后3天或7天，海马CA 1和CA 3区锥体细胞层未见明显变性迹象。此外，在CA 1或CA 3锥体细胞的形态特征没有观察到显着的变化与肾上腺切除。这些结果表明，齿状回颗粒细胞需要糖皮质激素的生存和维持正常形态，并建议颗粒细胞的形态和/或生存可能会经历不断的波动，响应昼夜节律或应激诱导的糖皮质激素水平的变化。
In order to determine whether short-term glucocorticoid manipulations influence the morphology and survival of neurons in the adult mammalian hippocampal formation, we performed quantitative analyses of Golgi-impregnated and Nissl-stained tissue from the brains of sham operated male rats, adrenalectomized male rats and adrenalectomized male rats which received corticosterone replacement. Three days after adrenalectomy, massive cell death, as detected by a dramatic increase in number of pyknotic cells, was observed in the granule cell layer of the dentate gyrus. By seven days following adrenalectomy, the numbers of pyknotic cells were even greater. Moreover, significant decreases in cross-sectional cell body area and numbers of dendritic branch points of Golgi-impregnated dentate gyrus granule cells were detected at seven days after adrenalectomy. Replacement of corticosterone to adrenalectomized rats prevented the appearance of large numbers of pyknotic cells as well as the decrease in granule cell cross-sectional cell body area and the numbers of dendritic branch points. In contrast, no obvious signs of degeneration were detedted in the pyramidal cell layers of the CA1 and CA3 regions of the hippocampus at either three or seven days following adrenalectomy. In addition, no significant changes in morphological characteristics were observed in CA1 or CA3 pyramidal cells with adrenalectomy. These results show that dentate gyrus granule cells require glucocorticoids for their survival and for the maintenance of normal morphology and suggest that granule cell morphology and/or survival may undergo constant fluctuation in response to diurnal rhythms or stress-induced changes in glucocorticoid levels.