Multiple receptors involved in human rhinovirus attachment to live cells
Multiple receptors involved in human rhinovirus attachment to live cells
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DOI：
10.1073/pnas.0806451105
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发表时间：
2008-11-18
影响因子：
11.1
通讯作者：
Hinterdorfer, Peter
中科院分区：
文献类型：
--
作者：
Rankl, Christian;Kienberger, Ferry;Hinterdorfer, Peter
Minor group human rhinoviruses (HRVs) attach to members of the low-density lipoprotein receptor family and are internalized via receptor-mediated endocytosis. The attachment of HRV2 to the cell surface, the first step in infection, was characterized at the single-molecule level by atomic force spectroscopy. Sequential binding of multiple receptors was evident from recordings of characteristic quantized force spectra, which suggests that multiple receptors bound to the virus in a timely manner. Unbinding forces required to detach the virus from the cell membrane increased within a time frame of several hundred milliseconds. The number of receptors involved in virus binding was determined, and estimates for on-rate, off-rate, and equilibrium binding constant of the interaction between HRV2 and plasma membrane-anchored receptors were obtained.
