The increased oxygen content in tantalum leads to decreased bioactivity and osteogenic ability of tantalum implants
The increased oxygen content in tantalum leads to decreased bioactivity and osteogenic ability of tantalum implants
复制标题
DOI：
10.1039/d0bm01555e
复制
发表时间：
2021-02-21
影响因子：
6.6
通讯作者：
Hu, Yihe
中科院分区：
文献类型：
--
作者：
Lei, Ting;Qian, Hu;Hu, Yihe
由于极高的熔点以及氧与钽的高亲和力，采用当前加工技术制造的钽（Ta）植入物不可避免地含有或多或少的氧杂质。因此，在本研究中，我们研究了氧杂质是否会对 Ta 的生物活性造成影响。 EDS分析表明，不同制备的Ta样品之间存在表面氧含量差异，高氧含量Ta（HO-Ta）的表面水接触角（WCA）显着高于中氧含量Ta（MO-Ta）和低氧含量Ta（LO-Ta）。体外细胞实验表明，MC3T3-E1细胞在氧含量较低的Ta上表现出更好的粘附、生长、形态发育和体外成骨能力。同样，体内动物实验表明，含氧量较低的Ta具有更好的骨再生和向内生长性能。此外，HO-Ta组中检测到ROS产量最高，而LO-Ta组中ROS产量最低。这项研究表明，由于技术限制，Ta 中的氧含量不可避免地出现，它以剂量依赖性方式对 Ta 的生物活性产生负面影响，这表明需要开发生产骨科全 Ta 植入物的技术。
Tantalum (Ta) implants fabricated by current processing techniques inevitably contain more or less oxygen impurities due to the extremely high melting point and high affinity of oxygen for Ta. Therefore, in this study we investigated whether oxygen impurities cause any effects on the bioactivity of Ta. EDS analysis demonstrated the surface oxygen content difference among different fabricated Ta samples, and the surface water contact angle (WCA) of Ta with high oxygen content (HO-Ta) was significantly higher than that of Ta with medium (MO-Ta) and low (LO-Ta) oxygen content. The in vitro cellular experiments showed that MC3T3-E1 cells on Ta with lower oxygen content exhibited better adhesion, growth, morphological development and in vitro osteogenic ability. Similarly, the in vivo animal experiments indicated the better bone regeneration and ingrowth performances of Ta with lower oxygen content. In addition, the highest ROS production was detected in the HO-Ta group, while the lowest in the LO-Ta group. This study suggests that the oxygen content within Ta, which occurs unavoidably due to technical limitations, negatively affects the bioactivity of Ta in a dose-dependent manner, indicating the need to develop techniques to produce orthopedic all-Ta implants.