Transformation of Cell-Derived Microparticles into Quantum-Dot-Labeled Nanovectors for Antitumor siRNA Delivery
Transformation of Cell-Derived Microparticles into Quantum-Dot-Labeled Nanovectors for Antitumor siRNA Delivery
复制标题
将细胞衍生的微粒转化为量子点标记的纳米载体以用于抗肿瘤 siRNA 递送
DOI：
10.1002/anie.201410223
复制
发表时间：
2015-01-12
影响因子：
16.6
通讯作者：
Zhao, Yi-Fang
中科院分区：
文献类型：
--
作者：
Chen, Gang;Zhu, Jun-Yi;Zhao, Yi-Fang
细胞来源的微粒（MP）最近已被认为是重要的细胞间的信息传递。然而，由于目前标记技术的局限性，对其生物学行为和潜在应用的进一步了解受到阻碍。在此，提出了一种通用的供体细胞辅助膜生物素化策略，通过巧妙地利用其供体细胞的天然膜磷脂交换来标记MP。这种创新策略方便地导致MP的特异性、高效、可再现和生物相容性量子点（QD）标记，从而可靠地赋予MP有价值的可追溯性。通过进一步负载小干扰RNA，具有固有的细胞靶向和生物分子输送能力的QD标记的MP被成功地用于组合生物成像和肿瘤靶向治疗。这项研究提供了第一个可靠的和生物友好的策略，将生物MP转化为功能化的纳米载体。
Cell-derived microparticles (MPs) have been recently recognized as critical intercellular information conveyors. However, further understanding of their biological behavior and potential application has been hampered by the limitations of current labeling techniques. Herein, a universal donor-cell-assisted membrane biotinylation strategy was proposed for labeling MPs by skillfully utilizing the natural membrane phospholipid exchange of their donor cells. This innovative strategy conveniently led to specific, efficient, reproducible, and biocompatible quantum dot (QD) labeling of MPs, thereby reliably conferring valuable traceability on MPs. By further loading with small interference RNA, QD-labeled MPs that had inherent cell-targeting and biomolecule-conveying ability were successfully employed for combined bioimaging and tumor-targeted therapy. This study provides the first reliable and biofriendly strategy for transforming biogenic MPs into functionalized nanovectors.