Alterations in histone acetylation following exposure to 60Co γ-rays and their relationship with chromosome damage in human lymphoblastoid cells
Alterations in histone acetylation following exposure to 60Co γ-rays and their relationship with chromosome damage in human lymphoblastoid cells
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暴露于 60Co γ 射线后组蛋白乙酰化的变化及其与人淋巴母细胞染色体损伤的关系
DOI：
10.1007/s00411-018-0742-9
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Qing-Jie Liu
中科院分区：
文献类型：
--
作者：
Xue-Lei Tian;Xue Lu;Jiang-Bin Feng;Tian-Jing Cai;Shuang Li;Mei Tian;Qing-Jie Liu
Chromosome damage is related to DNA damage and erroneous repair. It can cause cell dysfunction and ultimately induce carcinogenesis. Histone acetylation is crucial for regulating chromatin structure and DNA damage repair. Ionizing radiation (IR) can alter histone acetylation. However, variations in histone acetylation in response to IR exposure and the relationship between histone acetylation and IR-induced chromosome damage remains unclear. Hence, this study investigated the variation in the total acetylation levels of H3 and H4 in human lymphocytes exposed to 0-2 Gy60Co γ-rays. Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase (HDAC) inhibitor, was added to modify the histone acetylation state of irradiated cells. Then, the total acetylation level, enzyme activity, dicentric plus centric rings (dic + r) frequencies, and micronucleus (MN) frequencies of the treated cells were analyzed. Results indicated that the acetylation levels of H3 and H4 significantly decreased at 1 and 24 h, respectively, after radiation exposure. The acetylation levels of H3 and H4 in irradiated groups treated with SAHA were significantly higher than those in irradiated groups that were not treated with SAHA. SAHA treatment inhibited HDAC activity in cells exposed to 0-1 Gy60Co γ-rays. SAHA treatment significantly decreased dic + r/cell and MN/cell in cells exposed to 0.5 or 1.0 Gy60Co γ-rays relative to that in cells that did not receive SAHA treatment. In conclusion, histone acetylation is significantly affected by IR and is involved in chromosome damage induced by60Co γ-radiation.
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DOI：
10.1042/bj20080413
发表时间：
2009-02-01
期刊：
The Biochemical journal
影响因子：
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作者：
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通讯作者：
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DOI：
10.1158/1078-0432.ccr-10-0513
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期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
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作者：
Rossetto D;Truman AW;Kron SJ;Côté J
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Côté J
影响因子：
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影响因子：
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Zhang X;Kluz T;Gesumaria L;Matsui MS;Costa M;Sun H
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DOI：
10.1186/s13014-015-0455-z
发表时间：
2015-07-16
期刊：
Radiation oncology (London, England)
影响因子：
--
作者：
Blattmann C;Oertel S;Thiemann M;Dittmar A;Roth E;Kulozik AE;Ehemann V;Weichert W;Huber PE;Stenzinger A;Debus J
通讯作者：
Debus J