Control of leukocyte rolling velocity in TNF-α-induced inflammation by LFA-1 and Mac-1
Control of leukocyte rolling velocity in TNF-α-induced inflammation by LFA-1 and Mac-1
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DOI：
10.1182/blood.v99.1.336
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发表时间：
2002-01-01
期刊：
影响因子：
20.3
通讯作者：
Ley, K
中科院分区：
文献类型：
--
作者：
Dunne, JL;Ballantyne, CM;Ley, K
之前的研究表明，β(2)-整合素对于发炎小静脉中白细胞缓慢滚动是必需的。在这项研究中，使用缺乏 β (2)-整合素、α (L)β (2) (LFA-1) 或 α (M)β (2) (Mac-1) 之一的小鼠来确定哪种 β (2)-整合素负责减缓白细胞滚动。这些小鼠的提睾肌用肿瘤坏死因子-α处理并准备用于活体显微镜检查。与野生型小鼠 (4.8 +/- 0.3 mum/s) 相比，LFA-1(-/-) 小鼠 (111.0 +/- 0.7 mum/s) 和 Mac-1(-/-) 小鼠 (10.1 +/- 1.1 mum/s) 的小静脉平均滚动速度升高；P
Previously it was shown that beta (2)-integrins are necessary for slow leukocyte rolling in inflamed venules. In this study, mice that are deficient for either one of the beta (2)-integrins, alpha (L)beta (2) (LFA-1) or alpha (M)beta (2) (Mac-1), were used to determine which of the beta (2)-integrins are responsible for slowing rolling leukocytes. The cremaster muscles of these mice were treated with tumor necrosis factor-alpha and prepared for intravital microscopy. The average rolling velocities in venules were elevated in LFA-1(-/-) mice (111.0 +/- 0.7 mum/s) and Mac-1(-/-) mice (10.1 +/- 1.1 mum/s) compared to wild-type mice (4.8 +/- 0.3 mum/s; P