Morphine enhances complement receptor-mediated phagocytosis of Cyptococcus neoformans by human microglia
Morphine enhances complement receptor-mediated phagocytosis of Cyptococcus neoformans by human microglia
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DOI：
10.1006/clin.1998.4518
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发表时间：
1998-05-01
期刊：
影响因子：
--
通讯作者：
Peterson, PK
中科院分区：
文献类型：
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作者：
Lipovsky, MM;Gekker, G;Peterson, PK
最近的研究表明，阿片类药物可以调节小胶质细胞（大脑中常驻的巨噬细胞）的功能。在本研究中，研究了吗啡对人胎儿小胶质细胞吞噬机会性真菌新型隐球菌的影响。与猪小胶质细胞的早期发现相反，人类小胶质细胞吞噬新生隐球菌需要调理作用。此外，吗啡（10(-8) M）被证明可以使调理后的新型隐球菌的摄取增加50%以上。这与早期发现的吗啡诱导的猪小胶质细胞对非调理隐球菌吞噬作用的抑制作用形成鲜明对比。用μ阿片受体拮抗剂处理小胶质细胞以及向细胞培养物中添加抗补体受体抗体，可以逆转吗啡对人小胶质细胞隐球菌吞噬作用的影响，表明μ阿片受体和补体受体都参与吗啡增强的吞噬作用。这些发现支持阿片类药物作为神经免疫调节剂的概念，并证明阿片类药物对小胶质细胞的作用可能受到细胞来源动物物种的影响。 (C) 1998 年学术出版社。
Recent studies have shown that opiates modulate the function of microglia, the resident macrophages of the brain. In this study, the effect of morphine on phagocytosis by human fetal microglial cells of the opportunistic fungus Cryptococcus neoformans was studied. Contrary to earlier findings with swine microglia, opsonization was required for the phagocytosis of C. neoformans by human microglia. Moreover, morphine (10(-8) M) was shown to augment uptake of opsonized C. neoformans by over 50%. This contrasts with the earlier finding of morphine-induced inhibition of phagocytosis of nonopsonized cryptococci by swine microglia. The effect of morphine on cryptococcal phagocytosis by human microglia was reversed by treatment of microglial cells with mu, opiate receptor antagonists as well as by addition of anti-complement receptor antibodies to the cell cultures, indicating that both the mu opiate receptor and the complement receptor are involved in morphine-enhanced phagocytosis. These findings support the concept of opiates as neuroimmunomodulatory agents and demonstrate that the effects of opiates on microglial cells may be influenced by the animal species from which the cells are derived. (C) 1998 Academic Press.