Influence of tomato spotted wilt virus on performance and behaviour of western flower thrips (Frankliniella occidentalis)
Influence of tomato spotted wilt virus on performance and behaviour of western flower thrips (Frankliniella occidentalis)
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DOI：
10.1111/jen.12023
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发表时间：
2013-08-01
影响因子：
1.9
通讯作者：
Poehling, H. -M.
中科院分区：
文献类型：
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作者：
Ogada, P. A.;Maiss, E.;Poehling, H. -M.
昆虫病原体传播的一般原理涉及复杂而具体的相互作用，在这种情况下，蓟马、圆孢病毒及其共同宿主植物之间存在相互作用，这导致了具有经济和社会重要性的作物病害流行的爆发。通过在番茄斑萎病毒（TSWV）感染或未感染的辣椒小叶上饲养西花蓟马（WFT）整个幼虫阶段，部分研究了驱动其共同进化的可能过程和因素。随后，将蛹单独转移到健康的叶盘上，以进一步研究 TSWV 对 WFT 发育和行为模式的影响。研究发现，将 WFT 暴露于 TSWV 可以提高寿命和生存率，与未暴露的 WFT 相比，暴露于 TSWV 的 WFT 的平均寿命显着更高 (F-(3,F-403) = 22.44，P < 0.0001）。观察到的生存率改善是由于与未暴露者相比，暴露于 TSWV 的 WFT 个体死亡率显着降低 (F-(3,F-383) = 849.94，P < 0.0001）。然而，结果显示，四种处理期间平均每日繁殖力 (F-10,F-10) = 246.66，P < 0.0001）和终生繁殖力显着降低（F-(3,F-30) = 6.62，P = 0.001），以及终生繁殖力（F-(3,F-23) = 21.23，P < 0.0001）与未暴露于 TSWV 的 WFT 相比 未感染的叶盘。对于优先测试，与健康叶盘相比，感染 TSWV 的 C. annum 叶盘对 WFT 更具吸引力 (chi(2)((4, 34)) = 112.35，P < 0.0001）。这些结果预计将有助于清楚地了解植物-载体-病毒的相互作用，这对于准确诊断和控制 TSWV 流行以及控制作物害虫 F. occidentalis 至关重要。
The general principles in pathogen transmission by insects involve a complex and specific interplay, in this case between thrips, tospovirus and their shared host plant, which has led to outbreaks of crop disease epidemics of economic and social importance. The possible processes and factors driving their co-evolution were partly studied by rearing Frankliniella occidentalis [western flower thrips (WFT)] on either tomato spotted wilt virus (TSWV)-infected or uninfected Capsicum annum leaflets throughout their larval stages. Later, pupae were transferred individually on healthy leaf discs for further studies of the influence of TSWV on WFT development and behavioural patterns. The exposure of WFT to TSWV was found to improve performance with regard to longevity and survival, with mean longevity being significantly higher in TSWV-exposed WFT compared to unexposed ones (F-(3,F-403) = 22.44, P < 0.0001). The observed improvement in survival was as a result of significant reduction in mortality for the WFT individuals exposed to TSWV (F-(3,F-383) = 849.94, P < 0.0001) compared to the unexposed. However, the results showed a significant reduction in mean daily fecundity overtime (F-10,F-10) = 246.66, P < 0.0001) and across the four treatments (F-(3,F-30) = 6.62, P = 0.001), as well as lifetime fecundity (F-(3,F-23) = 21.23, P < 0.0001) of the WFT exposed to TSWV compared to the unexposed reared on uninfected leaf discs. For preferential test, C. annum leaf discs infected with TSWV were more attractive to WFT as compared to healthy leaf discs (chi(2)((4, 34)) = 112.35, P < 0.0001). These results are envisaged to contribute to a clear understanding into the plant-vector-virus interaction, which is essential for accurate diagnosis and control of the TSWV epidemic, as well as the control of F. occidentalis as crop pest.