SYNTHETIC BIOLOGY. On the record with E. coli DNA.
SYNTHETIC BIOLOGY. On the record with E. coli DNA.
复制标题
合成生物学。
DOI：
10.1126/science.aah4438
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Borkowski O
中科院分区：
文献类型：
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作者：
Borkowski O
合成生物学使用DNA程序为活细胞添加新功能。通过表达转录因子，像大肠杆菌这样的微生物可以执行复杂的计算逻辑，并在世代之间传递选定事件的“记忆”。但DNA不仅仅是蛋白质表达程序的源代码; DNA可以用作信息的存储介质。在合成生物学中，使用DNA进行体内信息存储是在2009年首次实现的，这是一个合成基因程序，它使E. colito通过使用重组酶来重新排列DNA以响应输入来计数事件。通过多个重组酶，这项技术可以在一个livingE中存储1.375字节的信息。杆菌正如Shipmanet等人在本期第463页和Roquetet等人所报道的那样，将信息在体内编码到DNA中的工作被进一步推进，要么使用基因组编辑来存储数十字节的数据，要么使用多个重组酶来实现活细胞内的“状态机”。
Synthetic biology uses DNA programs to add new functions into living cells. By expressing transcription factors, microbes such asEscherichia colican be made to perform complex computational logic and pass “memories” of selected events between generations . But DNA is more than just the source code for protein expression programs; DNA can be used as the storage medium for information. In synthetic biology, the use of DNA for in vivo information storage was first realized in 2009 with a synthetic genetic program that enabledE. colito count events by using a recombinase to rearrange DNA in response to an input . With multiple recombinases, this technology could be used to store 1.375 bytes of information in a livingE. coli. As reported by Shipmanet al.on page 463 of this issue, and by Roquetet al., in vivo encoding of information into DNA is pushed even further, using either genome editing to store dozens of bytes of data, or employing multiple recombinases to realize “state machines” inside living cells.