INTERACTION OF HUMAN ADENOVIRUS SEROTYPE-2 WITH HUMAN LYMPHOID-CELLS
INTERACTION OF HUMAN ADENOVIRUS SEROTYPE-2 WITH HUMAN LYMPHOID-CELLS
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DOI：
10.1016/0042-6822(88)90582-x
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发表时间：
1988-08-01
期刊：
影响因子：
3.7
通讯作者：
ANDERSON, CW
中科院分区：
文献类型：
--
作者：
SILVER, L;ANDERSON, CW
人腺病毒（例如，Ad2、Ad5)在人淋巴源性细胞系中建立慢性感染，包括 Raji 和 Jijoye (R. E. Wallace, 1969, Proc. Soc Exp. Biol. Med. 130, 702-710;N. Faucon. G. Ogier, and Y. Chardonnet, 1982, J. Natl. Cancer Inst. 69, 1215-1220）；然而，慢性感染产生和维持的机制尚不清楚。当 Raji 或 MOLT-3 细胞培养物以高复数感染 Ad2 时，这些细胞系继续呈指数增长，并且仅产生少量感染性病毒。病毒特异性抗原，包括 DNA 结合蛋白和六邻体，仅在 5% 的 Ad2 接种培养物中表达。所有 Raji 和 MOLT-3 细胞均被发现具有腺病毒受体，但吸附到大多数 Raji 细胞的 Ad2 病毒粒子被隔离在帽子中，表明大多数细胞无法内化吸附的 Ad2。细胞同步实验显示，有效感染的细胞比例与感染时处于有丝分裂状态的细胞比例之间存在相关性。相比之下，原代血液淋巴细胞几乎没有 Ad2 受体（如果有的话），并且不会被 Ad2 有效感染。
Human adenoviruses (e.g., Ad2, Ad5) establish chronic infections in human lymphoid-derived cell lines, including Raji and Jijoye (R. E. Wallace, 1969, Proc. Soc Exp. Biol. Med. 130, 702-710; N. Faucon. G. Ogier, and Y. Chardonnet, 1982, J. Natl. Cancer Inst. 69, 1215-1220); however, the mechanisms by which chronic infections are established and maintained are not understood. When Raji or MOLT-3 cell cultures were infected with Ad2 at high multiplicity, these cell lines continued to grow exponentially and produced only small amounts of infectious virus. Virus-specific antigens, including the DNA-binding protein and hexon, were expressed in only 5% of the Ad2-inoculated cultures. All Raji and MOLT-3 cells were found to have adenovirus receptors, but the Ad2 virions that adsorbed to most Raji cell were sequestered in caps, suggesting that most cells fail to internalize adsorbed Ad2. Cell synchronization experiments showed a correlation between the proportion of cells that became productively infected and the proportion of cells in mitosis at the time of infection. In contrast, primary blood lymphocytes had few, if any, Ad2 receptors and were not productively infected by Ad2.