The role of selenium in thyroid hormone action.
The role of selenium in thyroid hormone action.
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DOI：
10.1210/edrv-13-2-207
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发表时间：
1992-05
影响因子：
20.3
通讯作者：
M. Berry;P. R. Larsen
中科院分区：
文献类型：
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作者：
M. Berry;P. R. Larsen
I.甲状腺激素脱碘领域的最新进展不仅使人们对这一关键过程有了更深入的了解，而且也使人们对蛋白质合成的基本机制有了更深入的了解。I型脱碘酶的克隆，证明这种酶是一种硒蛋白，以及随后在基因的非编码部分中硒代半胱氨酸插入序列的鉴定，为将这种不寻常的氨基酸掺入蛋白质的机制提供了相当深入的了解。本文的目的是讨论其中的一些进展及其影响。我们开始通过提供一个简短的背景脱碘过程和所涉及的酶的发现，随后讨论了一些广泛的表征研究，这些酶已经进行了多年。对这些早期研究的全面综述已在之前发表（1，2）。我们接下来描述了I型脱碘酶的表达克隆及其鉴定。
I. Introduction RECENT progress in the field of thyroid hormone deiodination has led to a greater understanding, not only of this critical process, but also of mechanisms more basic to protein synthesis in general. The cloning of the type I deiodinase, demonstration that this enzyme is a selenoprotein, and subsequent identification of selenocysteine insertion sequences in the noncoding portion of the gene have provided considerable insight into the mechanism of incorporation of this unusual amino acid into proteins. The purpose of this review is to discuss some of these advances and their implications. We begin by providing a brief background of the deiodination process and the discovery of the enzymes involved, followed by a discussion of some of the extensive characterization studies that have been performed on these enzymes over the years. Comprehensive reviews of these earlier studies have been published previously (1, 2). We next describe the expression cloning of the type I deiodinase and its identi...