DETECTION OF THE SCHISTOSOME CIRCULATING CATHODIC ANTIGEN BY ENZYME-IMMUNOASSAY USING BIOTINYLATED MONOCLONAL-ANTIBODIES
DETECTION OF THE SCHISTOSOME CIRCULATING CATHODIC ANTIGEN BY ENZYME-IMMUNOASSAY USING BIOTINYLATED MONOCLONAL-ANTIBODIES
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DOI：
10.1016/0035-9203(90)90094-u
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发表时间：
1990-11-01
影响因子：
2.2
通讯作者：
DEELDER, AM
中科院分区：
文献类型：
--
作者：
DEJONGE, N;KREMSNER, PG;DEELDER, AM
We have developed an enzyme immunoassay (ELISA) for the quantification of the schistosome circulating cathodic antigen (CCA), a glycoprotein associated with the syncitium lining the gut of the parasite. A mouse monoclonal antibody of IgG3 isotype was used as coating (antigen-capture) antibody, while a biotinylated mouse monoclonal IgM was used as second (antigen-detecting) antibody. Streptavidin-alkaline phosphatase was used as enzyme label. The lower detection limit of the assay was 1.0 ng of the trichloroacetic acid soluble fraction of adult worm antigen (AWA-TCA) per ml, which corresponds to approximately 0.2 ng CCA per ml. The ELISA showed a linear range from 1.0 to 62.5 ng AWA-TCA per ml. Serum and urine samples of 16 individuals infected with Schistosoma mansoni (egg counts ranging from 5 to 4820 eggs per gram of faeces) were tested in the assay. Antigen titres ranged from < 4-8192. This assay represents a considerable advantage in diagnosis of Schistosoma infections as it allows the detection and quantification of CCA in serum and urine in even lightly infected individuals.
