Multi-stage chemical carcinogenesis in mouse skin: fundamentals and applications.
Multi-stage chemical carcinogenesis in mouse skin: fundamentals and applications.
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DOI：
10.1038/nprot.2009.120
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发表时间：
2009
期刊：
影响因子：
14.8
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中科院分区：
文献类型：
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60多年来，化学诱导小鼠皮肤肿瘤一直被用于研究上皮癌变机制和评价修饰因子。在传统的两阶段皮肤癌变模型中，起始是通过应用亚致癌剂量的致癌物来完成的。随后，肿瘤的发展是由肿瘤促进剂反复治疗引起的。根据使用的小鼠数量，起始方案可在1-3小时内完成，而推广阶段需要在研究期间每周治疗两次（1-2小时）和每周一次肿瘤触诊（1-2小时）。高度可再生的乳头状瘤负担预计在10-20周内，部分肿瘤在20-50周内进展为鳞状细胞癌。与完全的皮肤癌变相比，两阶段模型允许更多的癌前病变以及起始和促进阶段的分离。
For more than 60 years, the chemical induction of tumors in mouse skin has been used to study mechanisms of epithelial carcinogenesis and evaluate modifying factors. In the traditional two-stage skin carcinogenesis model, initiation is accomplished by the application of a subcarcinogenic dose of a carcinogen. Subsequently, tumor development is elicited by repeated treatment with a tumor promoting agent. The initiation protocol can be completed within 1–3 hours depending on the number of mice used, while the promotion phase requires twice weekly treatments (1–2 hours) and once weekly tumor palpation (1–2 hours) for the duration of the study. A highly reproducible papilloma burden is expected within 10–20 weeks with progression of a portion of the tumors to squamous cell carcinomas within 20–50 weeks. In contrast to complete skin carcinogenesis, the two-stage model allows for greater yield of premalignant lesions as well as separation of the initiation and promotion phases.