Construction by polymerase chain reaction and intragenic DNA probes for three main types of transferable β-lactamases (TEM, SHV, CARB)
Construction by polymerase chain reaction and intragenic DNA probes for three main types of transferable β-lactamases (TEM, SHV, CARB)
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通过聚合酶链反应和基因内 DNA 探针构建三种主要类型的可转移 β-内酰胺酶（TEM、SHV、CARB）
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
A. Philippon
中科院分区：
文献类型：
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作者：
G. Arlet;A. Philippon
以3种主要的β-内酰胺酶（TEM、SHV、CARB）基因片段为模板，通过聚合酶链反应合成基因内DNA探针。TEM探针与TEM-1、TEM-2和6种超广谱相关酶（TEM-3 ~ TEM-7、TEM-20）的编码基因进行菌落杂交和Southern-blot分析。SHV探针与SHV-1、俄亥俄州-1和4种衍生的超广谱β-内酰胺酶（SHV-2、SHV-3、SHV-4和SHV-5）的基因杂交。CARB探针与PSE-1（CARB-2）、PSE-4（CARB-1）、CARB-3和CARB-4的基因杂交。所有探针均未与8种苯唑西林水解酶（PSE-2、OXA-1至OXA-7、ROB-1和各种肠杆菌科（肺炎克雷伯菌除外）和铜绿假单胞菌的染色体β-内酰胺酶）的任何基因杂交。使用这些探针对大肠杆菌临床分离株进行的研究表明存在一种新型的超广谱、可转移的β-内酰胺酶。
Intragenic DNA probes were synthesized by polymerase chain reaction using fragments of the genes of three major types of β-lactamases (TEM, SHV, CARB) as templates. The TEM probe hybridized with the genes encoding TEM-1, TEM-2 and six extended-spectrum related enzymes (TEM-3 to TEM-7, TEM-20) in colony hybridizations and Southern-blot analysis. The SHV probe hybridized with the genes for SHV-1, OHIO-1 and four derived extended-spectrum β-lactamases (SHV-2, SHV-3, SHV-4 and SHV-5). The CARB probe hybridized with the genes for PSE-1 (CARB-2), PSE-4 (CARB-1), CARB-3 and CARB-4. None of the probes hybridized with genes for any of eight oxacillin-hydrolysing enzymes, PSE-2, OXA-1 to OXA-7, ROB-1 and chromosomal β-lactamases of various Enterobacteriaceae (except Klebsiella pneumoniae) and Pseudomonas aeruginosa. Investigations of Escherichia coli clinical isolates using these probes indicate the presence of a novel type of extended-spectrum, transferable β-lactamase.