Persistent Infection of Continuous Line of Pig Kidney Cells with a Variant of the WSN Strain of Influenza A0 Virus
Persistent Infection of Continuous Line of Pig Kidney Cells with a Variant of the WSN Strain of Influenza A0 Virus
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A0 型流感病毒 WSN 株变种对猪肾细胞连续系的持续感染
DOI：
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发表时间：
1972
期刊：
影响因子：
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通讯作者：
B. G. Tumyan
中科院分区：
文献类型：
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作者：
V. I. Gavrilov;D. Asher;S. D. Vyalushkina;L. S. Ratushkina;R. G. Zmieva;B. G. Tumyan
用流感A0病毒WSN株RES16变异株感染RES细胞，培养基频繁更换；建立了5个细胞系，这些细胞系的感染持续了126至146天。每个持续感染的细胞系都表现出细胞退化和再生的循环；然而，这些培养物并没有无限期地与病毒保持平衡，最终会退化。在不同的细胞系中，用尿囊内接种具有尿囊液凝集作用的卵作为指标，可以很容易地证明病毒在RES培养液中的存在，时间为27至105天。随后，这种方法未能证明病毒，尽管CPE的持续存在表明培养物仍被感染。用感染品系的液体接种正常RES或PS培养物，用CPE的产生作为病毒存在的指标，发现流感A0病毒仍然存在于液体中，并且能够在鸡蛋中繁殖而不产生可检测到的血凝素。在非血凝性尿囊液中仅发现适度的感染滴度；然而，具有相同感染滴度的原始WSN和WSN/RES16变体容易产生可检测到的血凝。这一事实表明，病毒并不是简单地失去了在鸡蛋中繁殖到足以产生血凝的滴度的能力，而是至少部分传染性病毒没有发生血凝。在感染的最后几天，就在培养物完全退化之前，在一个感染系（VL-V）中发现了自发恢复到原始血凝状态的现象，在两个病例中，恢复是在卵子（VL-V-63）和卵子和PS细胞（VL-IV-106）中连续传代后发生的。
Summary RES cells were infected with the RES16 variant of the WSN strain of influenza A0 virus and the culture medium changed at frequent intervals; five lines of cells were established in which infection was sustained for periods of 126 to 146 days. Each line of persistently-infected cells showed recurring cycles of cell degeneration and repopulation; these cultures did not remain in equilibrium with the virus indefinitely, however, and ultimately degenerated. The presence of virus in the RES culture fluids was easily demonstrated for periods of 27 to 105 days in various cell lines using as the indicator intraallantoic inoculation of eggs with hemagglutination by the allantoic fluids. Subsequently this method failed to demonstrate virus, although the continued presence of CPE suggested that the cultures were still infected. By using as indicator of the presence of virus the production of CPE in normal RES or PS cultures inoculated with fluids from the infected lines, it was found that influenza A0 virus was still present in the fluids, and that it was able to propagate in eggs without producing detectable hemagglutinins. Only modest infectivity titers were found in the nonhemagglutinating allantoic fluids; however the original WSN and WSN/RES16 variants at the same infectivity titers readily produced detectable hemagglutination. This fact suggests that the virus had not simply lost its ability to multiply in eggs to titers sufficient to produce hemagglutination, but that at least part of the infectious virus did not hemagglutinate. Spontaneous reversion to the original hemag-glutinating state was noted in one infected line (VL-V) during the last days of infection, just before complete degeneration of the culture, and in two cases reversion followed serial passages in eggs (VL-V-63) and in eggs and PS cells (VL-IV-106).