The antimicrobial mechanism of electrochemically activated water against Pseudomonas aeruginosa and Escherichia coli as determined by SDS-PAGE analysis
The antimicrobial mechanism of electrochemically activated water against Pseudomonas aeruginosa and Escherichia coli as determined by SDS-PAGE analysis
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DOI：
10.1111/j.1365-2672.2009.04233.x
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发表时间：
2009-08-01
影响因子：
4
通讯作者：
Kirkpatrick, R.
中科院分区：
文献类型：
--
作者：
Cloete, T. E.;Thantsha, M. S.;Kirkpatrick, R.
目的：暴露细菌的阳极电解液和随后的阳极电解液上的蛋白质的处理bacteries.Methods和结果：蛋白质提取与不同浓度的阳极电解液处理的细菌，并使用SDS-PAGE分析的影响。与未处理的细菌相比，在浓缩的卤化物阳极液处理的细菌中观察到更少和更微弱的蛋白条带，而在暴露于稀释浓度的细菌中观察到额外的蛋白条带。结论：未稀释的和10（-1）稀释的卤化物衍生的阳极液在杀死测试细菌方面是有效的。阳极电解液引起细菌死亡的蛋白质的完全破坏或引起氧化应激，导致蛋白质fragmentation.Significance和影响的研究：这项研究的结果提供了信息的抗菌机制的阳极电解液对其他细菌的信息是目前不可用的。
Aims: To expose bacteria to anolyte and subsequently investigate the effect of anolyte on the protein profiles of treated bacteria.Methods and Results: Proteins were extracted from bacteria treated with different concentrations of anolyte and analysed using SDS-PAGE. Fewer and more faint protein bands were observed for concentrated halide anolyte treated bacteria when compared to untreated bacteria while extra protein bands were observed for bacteria exposed to dilute concentrations.Conclusions: The undiluted and the 10(-1) dilution of halide derived anolyte was effective in killing the test bacteria. Anolyte caused bacterial death by complete destruction of proteins or by causing oxidative stress which resulted in protein fragmentation.Significance and Impact of the Study: The results of this study provide information on the antimicrobial mechanism of anolyte on other bacteria for which the information is currently unavailable.