Luteinizing hormone receptor, steroidogenesis acute regulatory protein, and steroidogenic enzyme messenger ribonucleic acids are overexpressed in thecal and granulosa cells from polycystic ovaries
Luteinizing hormone receptor, steroidogenesis acute regulatory protein, and steroidogenic enzyme messenger ribonucleic acids are overexpressed in thecal and granulosa cells from polycystic ovaries
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DOI：
10.1210/jc.86.3.1318
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发表时间：
2001-03-01
影响因子：
5.8
通讯作者：
Magoffin, DA
中科院分区：
文献类型：
--
作者：
Jakimiuk, AJ;Weitsman, SR;Magoffin, DA
最近的数据表明，类固醇生成酶信使核糖核酸（mRNA）可能在卵泡膜细胞中过度表达，LH受体可能在多囊卵巢妇女的颗粒细胞中过早表达。本研究的目的是确定是否有异常基因表达的卵泡膜细胞和颗粒细胞从多囊卵巢。卵巢组织标本来自12名PCOS妇女和24名定期骑自行车的对照妇女。从单个卵泡显微解剖颗粒细胞和内膜。通过RT-PCR测定LH受体、类固醇生成急性调节蛋白（星星）、胆固醇侧链裂解细胞色素P450（CYP 11 A）和17 α-羟化酶/C（17-20）裂解酶细胞色素P450（CYP 17）mRNA。卵泡膜细胞或颗粒细胞LH受体mRNA的表达在3- 7-mm的对照卵泡和优势卵泡之间没有差异。CYP 11 A和CYP 17 mRNA在3- 7 mm卵泡的卵泡膜细胞中高于优势卵泡，但星星表达没有差异。在颗粒细胞中，星星和CYP 11 A mRNA表达在优势卵泡中高于3- 7-mm卵泡。PCOS卵泡的卵泡膜细胞LH受体、星星、CYP 11 A和CYP 17 mRNA表达的平均水平高于大小匹配的对照卵泡。在颗粒细胞中，LH受体和CYP 11 A的平均水平，但不是星星，mRNA表达PCOS组高于对照组卵泡。这些数据表明，调节蛋白和类固醇生成酶mRNA在多囊卵巢的卵泡膜细胞和颗粒细胞中过表达，并支持卵泡膜细胞受到过度刺激且颗粒细胞可能过早黄体化的结论。
Recent data suggest that steroidogenic enzyme messenger ribonucleic acids (mRNAs) may be overexpressed in thecal cells, and LH receptors may be prematurely expressed in granulosa cells in women with polycystic ovaries. The purpose of this study was to determine whether there is abnormal gene expression in thecal and granulosa cells from polycystic ovaries. Ovarian tissue specimens were obtained from 12 women with PCOS and 24 regularly cycling control women. The granulosa cells and the theca interna were microdissected from individual follicles. LH receptor, steroidogenesis acute regulatory protein (StAR), cholesterol side-chain cleavage cytochrome P450 (CYP11A), and 17 alpha -hydroxylase/C(17-20) lyase cytochrome P450 (CYP17) mRNAs were measured by RT-PCR. There was no difference between 3- to 7-mm control follicles and dominant follicles with respect to LH receptor mRNA expression in either thecal or granulosa cells. CYP11A and CYP17 mRNAs were higher in thecal cells from 3- to 7-mm follicles than in dominant follicles, but StAR expression was not different. In granulosa cells, StAR and CYP11A mRNA expression was higher in dominant follicles than in 3- to 7-mm follicles. The mean levels of LH receptor, StAR, CYP11A, and CYP17 mRNA expression were higher in thecal cells from PCOS follicles than in size-matched control follicles. In granulosa cells, the mean levels of LH receptor and CYP11A, but not StAR, mRNA expression were higher in PCOS than in control follicles. These data demonstrate that regulatory protein and steroidogenic enzyme mRNAs are overexpressed in thecal and granulosa cells from polycystic ovaries and support the conclusions that the thecal cells are hyperstimulated and the granulosa cells may be prematurely luteinizing.