DNA index determination with Automated Cellular Imaging System (ACIS) in Barrett's esophagus: comparison with CAS 200.
DNA index determination with Automated Cellular Imaging System (ACIS) in Barrett's esophagus: comparison with CAS 200.
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DOI：
10.1186/1472-6890-5-7
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发表时间：
2005-08-12
影响因子：
--
通讯作者：
Goyal RK
中科院分区：
文献类型：
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作者：
Huang Q;Yu C;Klein M;Fang J;Goyal RK
对于实体瘤，图像细胞术对于诊断 DNA 含量异常（非整倍体）比流式细胞术更敏感。图像细胞计数通常使用半自动 CAS 200 系统进行。最近，引入了自动细胞成像系统（ACIS）来测定 DNA 含量（DNA 指数），但尚未得到验证。使用CAS 200系统和ACIS，我们比较了从巴雷特食管相关病变的相同存档的福尔马林固定和石蜡包埋的组织样本中获得的DNA指数（DI），包括具有特化肠化生但无不典型增生、低度不典型增生、高度不典型增生和腺癌的样本。尽管 ACIS 和 CAS 200 测定的 DI 值之间存在非常好的相关性，但前者在检测非整倍性方面的灵敏度高出 25%。 ACIS 产生的平均 DI 值比 CAS 200 获得的值高 18%（p < 0.001；配对 t 检验）。此外，使用 ACIS（30-40 分钟）执行 DNA 倍性分析所需的平均时间比使用 CAS 200（40-70 分钟）更短。 ACIS 获得的结果显示了出色的观察者间和观察者内变异性（两者的相关系数均 >0.9，p < 0.0001）。与 CAS 200 相比，ACIS 是一种更灵敏、更省时的 DNA 倍体测定技术。 ACIS 获得的结果也具有高度可重复性。
For solid tumors, image cytometry has been shown to be more sensitive for diagnosing DNA content abnormalities (aneuploidy) than flow cytometry. Image cytometry has often been performed using the semi-automated CAS 200 system. Recently, an Automated Cellular Imaging System (ACIS) was introduced to determine DNA content (DNA index), but it has not been validated. Using the CAS 200 system and ACIS, we compared the DNA index (DI) obtained from the same archived formalin-fixed and paraffin embedded tissue samples from Barrett's esophagus related lesions, including samples with specialized intestinal metaplasia without dysplasia, low-grade dysplasia, high-grade dysplasia and adenocarcinoma. Although there was a very good correlation between the DI values determined by ACIS and CAS 200, the former was 25% more sensitive in detecting aneuploidy. ACIS yielded a mean DI value 18% higher than that obtained by CAS 200 (p < 0.001; paired t test). In addition, the average time required to perform a DNA ploidy analysis was shorter with the ACIS (30–40 min) than with the CAS 200 (40–70 min). Results obtained by ACIS gave excellent inter-and intra-observer variability (coefficient of correlation >0.9 for both, p < 0.0001). Compared with the CAS 200, the ACIS is a more sensitive and less time consuming technique for determining DNA ploidy. Results obtained by ACIS are also highly reproducible.