Single immunization with a suboptimal antigen dose encapsulated into polyanhydride microparticles promotes high titer and avid antibody responses
Single immunization with a suboptimal antigen dose encapsulated into polyanhydride microparticles promotes high titer and avid antibody responses
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DOI：
10.1002/jbm.b.32820
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发表时间：
2013-01-01
影响因子：
3.4
通讯作者：
Ramer-Tait, Amanda E.
中科院分区：
文献类型：
--
作者：
Huntimer, Lucas;Welder, Jennifer H. Wilson;Ramer-Tait, Amanda E.
基于可生物降解聚酐的微粒佐剂用于向小鼠提供模型抗原卵清蛋白（Ova）的受控递送。将卵囊封入两种不同的聚酸酐微粒制剂中，以评价聚合物化学对次优剂量给药后体液免疫应答的性质和幅度的影响。皮下注射单剂量的含有25 μ g Ova的聚酸酐微粒引起的体液免疫应答与可溶性剂量的400 - 1600 μ g Ova引起的体液免疫应答的幅度相当。相比之下，与较高可溶性剂量相比，在施用微粒制剂的小鼠中，Ova特异性抗体的亲合力更大。最后，微粒递送系统引发了回忆性免疫应答，如当在接种后12周对小鼠施用25 μ g Ova的抗原攻击时，Ova特异性抗体的显著增加所证明的。总之，这些结果表明，将抗原包封到聚酸酐微粒中有利于同种型转换，建立免疫记忆，并且体液应答的特征在于更高质量的抗体应答。(c)2012 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 101 B：9198，2013.
Microparticle adjuvants based on biodegradable polyanhydrides were used to provide controlled delivery of a model antigen, ovalbumin (Ova), to mice. Ova was encapsulated into two different polyanhydride microparticle formulations to evaluate the influence of polymer chemistry on the nature and magnitude of the humoral immune response after administration of a suboptimal dose. Subcutaneous administration of a single dose of polyanhydride microparticles containing 25 mu g of Ova elicited humoral immune responses that were comparable in magnitude to that induced by soluble doses of 4001600 mu g Ova. In contrast, the avidity of the Ova-specific antibodies was greater in mice administered the microparticle formulations in comparison to the higher soluble doses. Finally, the microparticle delivery system primed an anamnestic immune response as evidenced by the significant increases in Ova-specific antibody when mice were administered an antigenic challenge of 25 mu g of Ova at 12 weeks post-vaccination. Together, these results indicate that encapsulation of antigens into polyanhydride microparticles facilitates isotype switching, establishes immunologic memory, and the humoral response was characterized by a higher quality antibody response. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 9198, 2013.