Surface antigens of malaria merozoites. A high molecular weight precursor is processed to an 83,000 mol wt form expressed on the surface of Plasmodium falciparum merozoites.
Surface antigens of malaria merozoites. A high molecular weight precursor is processed to an 83,000 mol wt form expressed on the surface of Plasmodium falciparum merozoites.
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DOI：
10.1084/jem.158.5.1647
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发表时间：
1983-11-01
影响因子：
15.3
通讯作者：
Holder, A A
中科院分区：
文献类型：
--
作者：
Freeman, R R;Holder, A A
研究了一种从寄生红细胞同步培养中获得高产量自然释放恶性疟原虫子体的技术。用胰蛋白酶处理培养的红细胞以防止再侵袭(6)，并通过差速离心收集细胞外积累的释放的分裂子。分析比较了分裂体和分裂子的总生物合成标记蛋白与人免疫血清免疫沉淀的总生物合成标记蛋白。还描述了乳酸过氧化物酶催化碘化标记的游离分裂子表面抗原。一种对195,000 mol wt分裂体蛋白特异的单克隆抗体及其衍生的加工片段(3)被用于免疫沉淀和Western转移分析，以确定哪些加工片段与分裂子有关，哪些片段位于分裂子表面。结果发现，195,000 mol wt的前体在游离的分裂子中被完整地加工成83,000 mol wt的片段，并且该片段作为恶性疟原虫分裂子的主要表面抗原之一表达。
A technique was developed for obtaining high yields of naturally released Plasmodium falciparum merozoites from synchronous cultures of parasitized erythrocytes. The cultured erythrocytes were treated with trypsin to prevent reinvasion (6), and the released merozoites that accumulated extracellularly were harvested by differential centrifugation. The total biosynthetically labeled proteins of schizonts and merozoites, and those immunoprecipitated by human immune serum were analyzed and compared. The surface antigens of free merozoites, labeled by lactoperoxidase-catalyzed iodination, were also described. A monoclonal antibody, specific for a 195,000 mol wt schizont protein, and processing fragments derived from it (3) were used in immunoprecipitation and Western transfer analyses to determine which of the processing fragments are associated with merozoites and which of them are located on the merozoite surface. It was found that processing of the 195,000 mol wt precursor down to an 83,000 mol wt fragment is complete in free merozoites, and that this fragment is expressed as one of the major surface antigens of P. falciparum merozoites.