TIIA attenuates LPS-induced mouse endometritis by suppressing the NF-κB signaling pathway
TIIA attenuates LPS-induced mouse endometritis by suppressing the NF-κB signaling pathway
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DOI：
10.1139/cjpp-2015-0003
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发表时间：
2015-11-01
影响因子：
2.1
通讯作者：
Cao, Rongfeng
中科院分区：
文献类型：
--
作者：
Lv, Xiaopei;Fu, Kaiqiang;Cao, Rongfeng
子宫内膜炎是危害奶牛业的主要疾病之一。丹参酮Ⅱ A（TIIA）是一种脂溶性生物碱，从丹参中分离得到，具有很强的抗炎作用。然而，TIIA对脂多糖（LPS）诱导的肠炎小鼠模型的抗炎作用仍有待阐明。本研究的目的是研究TIIA对LPS诱导的小鼠腹膜炎的影响。在LPS灌注子宫前1 h和灌注后12 h腹腔注射TIIA。然后进行组织学检查，并测定子宫组织中髓过氧化物酶（MPO）和一氧化氮（NO）的浓度。采用酶联免疫吸附法检测子宫组织匀浆中肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1 β）的水平。Western blotting检测I κ B α和p65的磷酸化程度。TIIA可明显减少中性粒细胞浸润，抑制MPO活性和NO浓度，降低TNF-α和IL-1 β的表达。此外，TIIA抑制核因子-κ B（NF-κ B）p65亚基的磷酸化及其抑制剂I κ B α的降解。以上结果提示TIIA对LPS诱导的小鼠腹膜炎有较强的抗炎作用。
Endometritis is one of the main diseases that harms the dairy cow industry. Tanshinone IIA (TIIA), a fat-soluble alkaloid isolated from Salviae miltiorrhizae, has been reported to have potent anti-inflammatory properties. However, the anti-inflammatory effects of TIIA on a mouse model of lipopolysaccharide (LPS)-induced endometritis remain to be elucidated. The purpose of the present study was to investigate the effects of TIIA on LPS-induced mouse endometritis. TIIA was intraperitoneally injected 1 h before and 12 h after perfusion of LPS into the uterus. A histological examination was then performed, and the concentrations of myeloperoxidase (MPO) and nitric oxide (NO) in the uterine tissue were determined. The levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in a homogenate of the uterus were detected by enzyme-linked immunosorbent assay. The extent of phosphorylation of I kappa B alpha and p65 was detected by Western blotting. TIIA markedly reduced the infiltration of neutrophils, suppressed MPO activity and the concentration of NO, and attenuated the expression of TNF-alpha and IL-1 beta. Furthermore, TIIA inhibited the phosphorylation of the nuclear factor-kappa B (NF-kappa B) p65 subunit and the degradation of its inhibitor I kappa B alpha. All the results suggest that TIIA has strong anti-inflammatory effects on LPS-induced mouse endometritis.