Genome sequence analysis of Ebola virus in clinical samples from three British healthcare workers, August 2014 to March 2015
Genome sequence analysis of Ebola virus in clinical samples from three British healthcare workers, August 2014 to March 2015
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DOI：
10.2807/1560-7917.es2015.20.20.21131
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发表时间：
2015-05-21
期刊：
影响因子：
19
通讯作者：
Zambon, M.
中科院分区：
文献类型：
--
作者：
Bell, A.;Lewandowski, K.;Zambon, M.
我们直接从临床样本中确定了三名在塞拉利昂感染埃博拉病毒 (EBOV) 的英国医护人员的完整病毒基因组序列。这些序列与之前在西非埃博拉病毒病爆发中观察到的序列非常相似，糖蛋白和聚合酶基因显示出最多的序列变异。我们的数据表明，目前的 PCR 诊断方法仍然适用于检测这种流行病中的埃博拉病毒，并为它们在诊断中的继续使用提供了信心。在西非埃博拉病毒病 (EVD) 持续爆发期间监测病毒基因组的进化对于早期检测可能逃避基于序列的诊断的突变体和监测治疗方案的疗效至关重要。我们在此介绍对埃博拉病毒 (EBOV) 序列的分析，这些序列是从塞拉利昂感染埃博拉病毒的三名英国医护人员 (HCW) 的血液样本中获得的。
We determined complete viral genome sequences from three British healthcare workers infected with Ebola virus (EBOV) in Sierra Leone, directly from clinical samples. These sequences closely resemble those previously observed in the current Ebola virus disease outbreak in West Africa, with glycoprotein and polymerase genes showing the most sequence variation. Our data indicate that current PCR diagnostic assays remain suitable for detection of EBOV in this epidemic and provide confidence for their continued use in diagnosis.Monitoring of the evolution of the viral genome during the ongoing outbreak of Ebola virus disease (EVD) in West Africa is crucial for the early detection of mutants that may evade sequence-based diagnostics and for monitoring efficacy of therapeutic options. We present here our analysis of Ebola virus (EBOV) sequences obtained from blood samples from three British healthcare workers (HCWs) who were infected with EBOV in Sierra Leone.