UP-REGULATION OF SURFACE-MARKERS ON DYING THYMOCYTES
UP-REGULATION OF SURFACE-MARKERS ON DYING THYMOCYTES
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DOI：
10.1084/jem.181.2.649
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发表时间：
1995-02-01
影响因子：
15.3
通讯作者：
SPRENT, J
中科院分区：
文献类型：
--
作者：
KISHIMOTO, H;SURH, CD;SPRENT, J
利用末端脱氧核苷酸转移酶介导的dutp -生物素nick end标记（TUNEL）检测早期凋亡细胞，我们定义了组织培养中自发或类固醇诱导凋亡的CD4(+)CD8（+）胸腺细胞上表达的表面标记物。一些表面标记物，如CD4、CD8和热稳定抗原，在凋亡的胸腺细胞上下调。然而，令人惊讶的是，其他标记上调；这适用于T细胞受体β /CD3、CD69和CD25的表达。这些标记的上调仅限于凋亡细胞的一个离散子集。
Using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) to detect cells undergoing early apoptosis, we have defined the surface markers expressed on CD4(+)CD8(+) thymocytes undergoing spontaneous or steroid-induced apoptosis in tissue culture. Some surface markers, e.g., CD4, CD8, and heat stable antigen, are downregulated on apoptotic thymocytes. Surprisingly, however, other markers are upregulated; this applies to T cell receptor beta/CD3, CD69, and CD25 expression. Upregulation of these markers is restricted to a discrete subset of apoptotic cells.