Chemical studies of partially hydrolysed lipopolysaccharides from four strains of Campylobacter jejuni and two strains of Campylobacter coli.
Chemical studies of partially hydrolysed lipopolysaccharides from four strains of Campylobacter jejuni and two strains of Campylobacter coli.
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来自四种空肠弯曲杆菌菌株和两种大肠杆菌菌株的部分水解脂多糖的化学研究。
DOI：
10.1099/00221287-130-11-2783
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
T. Hofstad
中科院分区：
文献类型：
--
作者：
V. Naess;T. Hofstad
用1%的乙酸对4株空肠弯曲杆菌和2株大肠杆菌的脂多糖进行部分水解。随后的氯仿提取导致形成含多糖的水层、界面物质和含脂a的氯仿层。多糖含有中性糖、氨基糖、2-酮-3-脱氧辛氨酸和部分未降解LPS中的磷。脂质As由葡萄糖胺、磷、酯和酰胺连接的3-羟基十四烷酸、酯连接的正十四烷酸和正十六烷酸组成。界面材料由脂质A和未降解的LPS组成。在生物凝胶P-60层析时，降解多糖被洗脱为两个不完全分离的峰（菌株NCTC 11168、NCTC 11351、11041和11101）或一个峰（菌株NCTC 11392和e8035）。所有峰均接近色谱柱的总体积。当不同组分在Bio-GEl P-10上重新色谱时，峰仍然出现在接近柱总体积的位置。这些结果表明，来自空肠C.和大肠C.的LPS缺乏长o抗原侧链。
Lipopolysaccharides (LPS) from four strains of Campylobacter jejuni and two strains of C. coli were partially hydrolysed with 1% acetic acid. Subsequent chloroform extraction led to the formation of a polysaccharide-containing aqueous layer, an interfacial material and a lipid A-containing chloroform layer. The polysaccharides contained the neutral sugars, amino sugars, 2-keto-3-deoxy-octonic acid, and part of the phosphorus present in the undegraded LPS. The lipid As were made up of glucosamine, phosphorus, ester- and amide-linked 3-hydroxytetradecanoic acid, and ester-linked n-tetradecanoic and n-hexadecanoic acid. The interfacial material was made up of lipid A and undegraded LPS. When chromatographed on Bio-GEl P-60, the degraded polysaccharides were eluted as two incompletely separated peaks (strains NCTC 11168, NCTC 11351, 11041 and 11101) or as one peak (strains NCTC 11392 and E 8035). All peaks appeared close to the total volume of the column. When the different fractions were re-chromatographed on Bio-GEl P-10, the peaks still appeared close to the total volume of the column. These findings indicate that LPS from C. jejuni and C. coli are devoid of long O-antigenic side-chains.