YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coli
YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coli
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DOI：
10.1038/s41467-019-13764-4
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发表时间：
2019-12-17
影响因子：
16.6
通讯作者：
Maisonneuve, Etienne
中科院分区：
文献类型：
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作者：
Germain, Elsa;Guiraud, Paul;Maisonneuve, Etienne
严格反应是一种一般的细菌应激反应，允许细菌适应并在不利条件下生存。这种细胞生理学的重编程是由alarmone（p）ppGpp的积累引起的，在大肠杆菌中，alarmone（p）ppGpp依赖于（p）ppGpp合成酶ReIA和双功能（p）ppGpp合成酶/水解酶SpoT。尽管已经描述了控制SpoT依赖性（p）ppGpp积累的条件，但是调节从（p）ppGpp降解到合成的转换的分子机制仍然知之甚少。在这里，我们发现蛋白YtfK促进了E.在磷酸盐和脂肪酸饥饿过程中激活严格反应所必需的。我们的研究结果表明，YtfK可以与SpoT相互作用。我们建议，YtfK激活严格的反应，通过倾斜的催化平衡点向（p）ppGpp合成。
The stringent response is a general bacterial stress response that allows bacteria to adapt and survive adverse conditions. This reprogramming of cell physiology is caused by the accumulation of the alarmone (p)ppGpp which, in Escherichia coli, depends on the (p)ppGpp synthetase ReIA and the bifunctional (p)ppGpp synthetase/hydrolase SpoT. Although conditions that control SpoT-dependent (p)ppGpp accumulation have been described, the molecular mechanisms regulating the switching from (p)ppGpp degradation to synthesis remain poorly understood. Here, we show that the protein YtfK promotes SpoT-dependent accumulation of (p)ppGpp in E. coli and is required for activation of the stringent response during phosphate and fatty acid starvation. Our results indicate that YtfK can interact with SpoT. We propose that YtfK activates the stringent response by tilting the catalytic balance of SpoT toward (p)ppGpp synthesis.