Development of microsatellite markers from four Pittosporum taxa (Pittosporaceae) endemic to the Ogasawara (Bonin) Islands with cross-amplification in Ogasawara Pittosporum and P. tobira
Development of microsatellite markers from four Pittosporum taxa (Pittosporaceae) endemic to the Ogasawara (Bonin) Islands with cross-amplification in Ogasawara Pittosporum and P. tobira
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开发小笠原群岛特有的四种海桐类群（海桐科）的微卫星标记，并在小笠原海桐和海桐中进行交叉扩增
DOI：
10.1007/s12686-012-9764-9
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
N. & Kato H.
中科院分区：
文献类型：
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作者：
Katoh;S.;Ohi-Toma;T.;Murakami;N. & Kato H.
利用日本小笠原群岛特有的4个pittosporumtaxa （Pittosporum boninensevar、boninense、P.boninensevar）共开发了55个微卫星位点。chichijimense,海桐parvifoliumvar.parvifoliumandP.parvifoliumvar.beecheyi。并对这些标记在OgasawaraPittosporumtaxa和aspittosporumtobira之间的可转移性进行了评价。每个基因座的等位基因数在2 ~ 23个之间。boninense， 2到16英寸。赤柱状茎，从1到7具小叶柄。小叶，1 - 8英寸的山毛榉。在交叉扩增试验中，这些基因座中的大多数被成功扩增，并显示出中等至高水平的遗传变异。本文所描述的标记可用于更准确地确定其来源分类群的遗传多样性和遗传结构。
A total of 55 microsatellite loci were developed and characterized using fourPittosporumtaxa endemic to the Ogasawara (Bonin) Islands, Japan:Pittosporum boninensevar.boninense,P.boninensevar.chichijimense,Pittosporum parvifoliumvar.parvifoliumandP.parvifoliumvar.beecheyi. Transferability of these markers among the OgasawaraPittosporumtaxa as well asPittosporumtobirawas also evaluated. The number of alleles per locus ranged from 2 to 23 inP.boninensevar.boninense, from 2 to 16 inP.boninensevar.chichijimense, from 1 to 7 with inP.parvifoliumvar.parvifolium, and from 1 to 8 inP.parvifoliumvar.beecheyi. During cross-amplification tests, the majority of these loci were successfully amplified and showed moderate to high levels of genetic variation. The markers described here could be used to achieve a more accurate determination of genetic diversity and genetic structure of the taxa from which they were derived.