Use of the enzyme-linked immunoadsorbent assay to monitor the purification of glycosphingolipid antigens by high-performance liquid chromatography.
Use of the enzyme-linked immunoadsorbent assay to monitor the purification of glycosphingolipid antigens by high-performance liquid chromatography.
复制标题
使用酶联免疫吸附测定法监测高效液相色谱法鞘糖脂抗原的纯化情况。
DOI：
10.1016/0003-2697(87)90527-6
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发表时间：
1987
影响因子：
2.9
通讯作者：
Macher,BA
中科院分区：
文献类型：
--
作者：
Buehler,J;Galili,U;Macher,BA
应用酶联免疫吸附测定（ELISA）技术对高效液相色谱分离的鞘糖脂组分进行了分析。所选组分的纳微克量放置在微滴孔中，使用亲和素-生物素酶系统（ABC试剂）分析携带单克隆抗体识别的碳水化合物表位的鞘糖脂。大量的分数（超过100）可以方便地评估鞘糖脂的存在，在一次分析中被一种或多种单克隆抗体识别。该方法是一种快速、灵敏的监测鞘脂抗原纯化的方法，可与薄层色谱分离的鞘脂免疫染色结合使用。
An enzyme-linked immunoadsorbent assay (ELISA) technique has been applied to the analysis of glycosphingolipid fractions separated by high-performance liquid chromatography. Nanogram amounts of selected fractions were placed in microtiter wells and analyzed for glycosphingolipids carrying carbohydrate epitopes recognized by monoclonal antibodies using an avidin-biotin enzyme system (ABC reagents). A large number of fractions (more than 100) can be conveniently evaluated for the presence of glycosphingolipids recognized by one or more monoclonal antibodies in a single analysis. This method is a rapid and sensitive procedure for monitoring the purification of glycosphingolipid antigens and can be used in conjunction with immunostaining of glycosphingolipids separated by thin-layer chromatography.