The prognostic roles of circulating ALDH1+ tumor cell in the patients with non-small cell lung cancer
The prognostic roles of circulating ALDH1+ tumor cell in the patients with non-small cell lung cancer
复制标题
循环ALDH1肿瘤细胞在非小细胞肺癌患者预后中的作用
DOI：
10.1042/bsr20180914
复制
发表时间：
2018-10-31
影响因子：
4
通讯作者：
Xia，Pu
中科院分区：
文献类型：
--
作者：
Tian，Shuang;Xing，Ya-Nan;Xia，Pu
非小细胞肺癌（non-small cell lung cancer，NSCLC）患者循环肿瘤细胞的检测可为NSCLC患者的诊断和预后提供重要信息。乙醛脱氢酶1（ALDH 1）是一种癌症干细胞标志物，已用于各种肿瘤，包括NSCLC。在本研究中，我们使用ALDH 1作为潜在的标记物从NSCLC患者中分离循环ALDH 1+肿瘤细胞。与正常对照组相比，在NSCLC患者的血液样品中鉴定出更高百分比的ALDH 1+肿瘤细胞。Kaplan-Meier分析显示ALDH 1+细胞与患者预后不良相关。最后，采用体外成瘤实验和小鼠移植瘤模型，对ALDH 1+肿瘤细胞的致瘤性进行了研究。我们的体外和体内实验表明，ALDH 1可以驱动循环NSCLC细胞的干细胞化。循环ALDH 1+细胞可作为NSCLC的预后标志物。
Circulating tumor cells can provide important diagnostic and prognostic information of the patients with non-small cell lung cancer (NSCLC). Aldehyde dehydrogenase 1 (ALDH1), a cancer stem cell marker, has been used in various tumors, including NSCLC. In the present study, we isolated the circulating ALDH1+tumor cells from the NSCLC patients using ALDH1 as a potential marker. Higher percentage of ALDH1+tumor cells was identified in blood samples from the NSCLC patients compared with normal controls. ALDH1+cells were correlated with the poor prognosis of these patients by using Kaplan–Meier analysis. In the last, the tumorigenic properties of ALDH1+tumor cells were determinedin vitroandin vivoby using sphere assay and xenograft tumor mouse models. Ourin vitroandin vivoexperiments demonstrated that ALDH1 could drive the stemness of circulating NSCLC cells. Circulating ALDH1+cells could be used as a prognostic marker for NSCLC.