Rapid Multiplex PCR Assay for the Simultaneous Detection of the Brucella Genus, B. abortus, B. melitensis, and B. suis
Rapid Multiplex PCR Assay for the Simultaneous Detection of the Brucella Genus, B. abortus, B. melitensis, and B. suis
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DOI：
10.4014/jmb.1007.07051
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Kumar, Om
中科院分区：
文献类型：
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作者：
Kumar, Sanjay;Tuteja, Urmil;Kumar, Om
布鲁氏菌属的常规鉴定和区分是一个耗时且劳动密集型的过程，这经常使工作人员处于实验室获得性感染的风险中。在这里，我们描述了一种快速多重PCR检测的发展，用于确认推定的布鲁氏菌分离株。该检测能够识别和区分主要的人类病原体，即B。abortus，牛种B. melitensis和B. suis，in a single单test测试of less下a half半hour小时and a half半.
The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratory-acquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.