Teratocarcinoma derived from mouse zygotes after the introduction of activated human c-Ha-ras DNA.
Teratocarcinoma derived from mouse zygotes after the introduction of activated human c-Ha-ras DNA.
复制标题
引入活化的人 c-Ha-ras DNA 后，源自小鼠受精卵的畸胎癌。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
S. Nishimura
中科院分区：
文献类型：
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作者：
M. Katsuki;M. Kimura;M. Yokoyama;M. Sato;S. Kimura;J. Hata;T. Sekiya;M. Izawa;S. Nishimura
我们试图生产转基因小鼠携带正常或激活的人类c-Ha-ras基因，以检查激活的癌基因在肿瘤发生中的功能。在发育过程中，不仅获得了外观正常的胎儿，而且获得了畸形儿、发育停滞的孕体和肿瘤。发现六个这样的异常发育的胚胎已与激活的人c-Ha-ras基因整合。这样获得的两个肿瘤之一是在注射含有p21基因的6.3kb DNA片段后形成的，该基因在第12位具有缬氨酸。该肿瘤在染色体中的特定位点处与引入的DNA的两个拷贝整合，并且以首尾方向串联排列。组织学分析显示，该肿瘤由至少三种类型的细胞构成：两种来自不同的胚层（一个内胚层和另一个中胚层），第三种来自胚外外胚层。另一个肿瘤显示出类似的特征。因此，这是强烈建议，这些肿瘤是来自非常早期的胚胎发育阶段，并具有非常相似的畸胎癌的功能。
We attempted to produce transgenic mice harboring the normal or activated human c-Ha-ras gene in order to examine the function of activated oncogenes in tumorigenesis. During the process of development, it happened that not only normal looking fetuses were obtained but also malformants, developmentally arrested conceptuses and tumors. Six such abnormally developed embryos were found to have been integrated with the activated human c-Ha-ras gene. One of the two tumors thus obtained was formed after the injection of 6.3 kb DNA fragment containing the gene for p21 with valine at the twelfth position. This tumor was integrated with two copies of the introduced DNA at a particular site in a chromosome and arranged in tandem in a head to tail direction. Histological analysis revealed that this tumor was constructed from at least three types of cells: two originating from different germ layers (one endoderm and the other mesoderm) and the third from an extra-embryonic ectoderm. The other tumor revealed similar features. Thus, it was strongly suggested that these tumors were derived from the very early developmental stage of the embryo, and had features very similar to those of teratocarcinoma.