Molecular interaction between Methylobacterium extorquens and seedlings:: growth promotion, methanol consumption, and localization of the methanol emission site
Molecular interaction between Methylobacterium extorquens and seedlings:: growth promotion, methanol consumption, and localization of the methanol emission site
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DOI：
10.1093/jxb/erl173
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发表时间：
2006-12-01
影响因子：
6.9
通讯作者：
Schwab, Wilfried
中科院分区：
文献类型：
--
作者：
Abanda-Nkpwatt, Daniel;Muesch, Martina;Schwab, Wilfried
从草莓（Fragariaxananassa cv. Elsanta）叶子，并且测试了一种称为ME 4的菌株促进各种植物幼苗生长的能力。幼苗的重量和芽长的烟草，番茄，白芥，草莓的粉红色的兼性甲基营养菌（PPFM）的存在下显着增加，但从其他6种植物的种子的萌发行为没有受到影响。细菌培养物的无细胞上清液刺激萌发，表明甲基营养菌产生了生长促进剂。N.通过质子转移反应质谱（PTR-MS）测定，烟草幼苗的挥发性醇含量范围为0.4至0.7 ppbv（十亿分之一体积），而当幼苗与M. extrorquens ME 4，表明细菌消耗气态甲醇。此外，通过使用用pPICHS/GFP载体转化的甲醇营养型酵母巴斯德毕赤酵母细胞，所述pPICHS/GFP载体含有甲醇敏感启动子和绿色荧光蛋白（GFP）报告基因的组合，气孔被鉴定为烟草子叶上甲醇排放的主要来源。扭脱甲基杆菌菌株可以利用气孔释放的甲醇滋养自身，并释放促进某些作物幼苗生长的试剂。
Four Methylobacterium extorquens strains were isolated from strawberry (Fragariaxananassa cv. Elsanta) leaves, and one strain, called ME4, was tested for its ability to promote the growth of various plant seedlings. Seedling weight and shoot length of Nicotiana tabacum, Lycopersicon esculentum, Sinapis alba, and Fragaria vesca increased significantly in the presence of the pink-pigmented facultative methylotroph (PPFM), but the germination behaviour of seeds from six other plants was not affected. The cell-free supernatant of the bacterial culture stimulated germination, suggesting the production of a growth-promoting agent by the methylotroph. Methanol emitted from N. tabacum seedlings, as determined by proton-transfer-reaction mass spectrometry (PTR-MS), ranged from 0.4 to 0.7 ppbv (parts per billion by volume), while significantly lower levels (0.005 to 0.01 ppbv) of the volatile alcohol were measured when the seedlings were co-cultivated with M. extorquens ME4, demonstrating the consumption of the gaseous methanol by the bacteria. Additionally, by using cells of the methylotrophic yeast Pichia pastoris transformed with the pPICHS/GFP vector harbouring a methanol-sensitive promoter in combination with the green fluorescence protein (GFP) reporter gene, stomata were identified as the main source of the methanol emission on tobacco cotyledons. Methylobacterium extorquens strains can nourish themselves using the methanol released by the stomata and release an agent promoting the growth of the seedlings of some crop plants.