Regional assignment of the erythropoietin gene to human chromosome region 7pter----q22.
Regional assignment of the erythropoietin gene to human chromosome region 7pter----q22.
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促红细胞生成素基因的区域分配到人类染色体区域7pter----q22。
DOI：
10.1159/000132281
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Shows,TB
中科院分区：
文献类型：
--
作者：
Watkins,PC;Eddy,R;Hoffman,N;Stanislovitis,P;Beck,AK;Galli,J;Vellucci,V;Gusella,JF;Shows,TB
通过Southern印迹杂交分析一组人-鼠体细胞杂交细胞DNA，确定了人促红细胞生成素（EPO）基因的染色体定位。用限制性内切酶Pst Ⅰ处理含有减少的人类染色体的杂交细胞的DNA，用克隆的人EPO cDNA探针进行筛选，在所有45个检测的杂交细胞中，EPO与人类7号染色体完全共分离，据此将EPO定位于该染色体。含有7号染色体易位衍生物的细胞杂种将EPO定位于7 pter →q22。通过EPO cDNA探针与人基因组DNA的杂交检测AHind III限制性片段长度多态性。
The chromosomal location of the human gene for erythropoietin(EPO) was determined by Southern blot hybridization analysis of a panel of human-mouse somatic hybrid cell DNAs. DNAs from cell hybrids containing reduced numbers of human chromosomes were treated with the restriction enzymePstIand screened with a cloned human EPO cDNA probe.EPOis assigned to human chromosome 7 based on the complete cosegregation ofEPOwith this chromosome in all 45 cell hybrids tested. A cell hybrid containing a translocated derivative of chromosome 7 localizesEPOto 7pter→q22. AHindIII restriction fragment length polymorphism is detected by hybridization of the EPO cDNA probe to human genomic DNA.