Enoxacin-Tb3+ complex as an environmentally friendly fluorescence probe for DNA and its application.
Enoxacin-Tb3+ complex as an environmentally friendly fluorescence probe for DNA and its application.
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DOI：
10.1016/j.talanta.2006.05.046
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发表时间：
2007-02
期刊：
影响因子：
6.1
通讯作者：
C. Tong;Zhoujian Hu;Wei-ping Liu
中科院分区：
文献类型：
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作者：
C. Tong;Zhoujian Hu;Wei-ping Liu
研究了DNA增强依诺沙星(ENX) -Tb3 +复合物的荧光强度。在此基础上，研制了一种环境友好的enoxacin-Tb3 +荧光探针，用于检测单链和双链DNA。在最佳条件下，增强的荧光强度与DNA浓度成正比，hsDNA为2.0×10−8 ~ 2.0×10−6gmL−1，ctDNA为1.0×10−8 ~ 1.0×10−6gmL−1，热变性ctDNA为5.0×10−9 ~ 1.0×10−6gmL−1。检出限（S/N=3）分别为5.0、9.0和3.0ngmL−1。详细讨论了ENX-Tb3 +与DNA的相互作用模式和荧光增强机理。紫外吸收光谱、荧光光谱以及ENX-Tb3 +配合物与EB探针的竞争组合实验结果表明，ENX-Tb3 +配合物与DNA之间可能存在至少两种不同的结合模式：静电结合和插层结合。此外，该荧光探针还用于研究重金属与DNA的相互作用。
The fluorescence intensity of the enoxacin (ENX)–Tb3+complex enhanced by DNA was studied. On the basis of this study, an environmentally friendly fluorescence probe of enoxacin–Tb3+for the determination of single-stranded and double-stranded DNA was developed. Under the optimal conditions, the enhanced fluorescence intensity was in proportion to the concentration of DNA in the range of 2.0×10−8to 2.0×10−6gmL−1for hsDNA, 1.0×10−8to 1.0×10−6gmL−1for ctDNA and 5.0×10−9to 1.0×10−6gmL−1for thermally denatured ctDNA. The detection limits (S/N=3) were 5.0, 9.0 and 3.0ngmL−1, respectively. The interaction modes between ENX–Tb3+and DNA and the mechanism of the fluorescence enhancement were also discussed in details. The experimental results from UV absorption spectra, fluorescence spectra and the competing combination tests between the ENX–Tb3+complex and EB probe indicated that the possible interaction modes between enoxacin–Tb3+complex and DNA had at least two different binding modes: the electrostatic binding and the intercalation binding. Additionally, this fluorescence probe was used to study the interaction between heavy metals and DNA.