Post-genomic approach based discovery of alkylresorcinols from a cricket-associated fungus, Penicillium soppi
Post-genomic approach based discovery of alkylresorcinols from a cricket-associated fungus, Penicillium soppi
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DOI：
10.1039/c9ob00807a
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发表时间：
2019-06-07
影响因子：
3.2
通讯作者：
Asai, Teigo
中科院分区：
文献类型：
--
作者：
Kaneko, Akiho;Morishita, Yohei;Asai, Teigo
在蟋蟀相关真菌Penicillium soppi中，聚酮合酶（PKS）基因引导的基因组挖掘揭示了一个隐藏的生物合成基因簇，其包含高度还原的PKS（HR-PKS）、III型PKS和P450基因。该簇在曲霉中的异源表达导致分离出具有独特Z，E，Z-三烯基序的新型烷基间苯二酚。这项研究显示了一个不寻常的生物合成机制的HR-PKS和一个新的释放机制，通过III型PKS在真菌中。
Polyketide synthase (PKS) gene-guided genome mining in a cricket-associated fungus, Penicillium soppi, revealed a cryptic biosynthetic gene cluster that contained a highly reducing PKS (HR-PKS), a type III PKS, and a P450 gene. Heterologous expression of the cluster in Aspergillus oryzae led to the isolation of novel alkylresorcinols with a unique Z,E,Z-triene motif. This study displays an unusual biosynthetic mechanism of an HR-PKS and a new releasing mechanism via a type III PKS in fungi.