Oligodendrocytes express P2Y12 metabotropic receptor in adult rat brain
Oligodendrocytes express P2Y12 metabotropic receptor in adult rat brain
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DOI：
10.1016/j.neuroscience.2006.05.058
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Volonte, C.
中科院分区：
文献类型：
--
作者：
Amadio, S.;Tramini, G.;Volonte, C.
在CNS中，在神经元和神经胶质细胞上鉴定出称为P2受体的核苷酸受体，其介导神经元-神经元、神经胶质-神经胶质和神经胶质-神经元通信。在本工作中，我们在体内的成年大鼠中枢神经系统的P2 Y（12）受体蛋白的细胞/亚细胞分布，在大脑皮质，白色物质和皮质下核（纹状体和黑质），通过免疫荧光共聚焦，电子显微镜和Western印迹分析。P2 Y（12）受体免疫反应性既不与标记物如神经元核、神经丝轻链、钙结合蛋白和酪氨酸羟化酶共定位，也不与胶质细胞酸性蛋白和同种凝集素B4共定位，而是与髓鞘碱性蛋白和少突胶质细胞标记物RIP共定位，在细胞体和突起中，因此指示少突胶质细胞定位。电子显微镜鉴定P2 Y（12）受体在核周和质膜下的少突胶质细胞体和辐射过程，直到纤维的旁结区。通过Western blot分析，P2 Y（12）受体显示42-44 kDa的特异性条带，与氨基酸测序预测的分子量相匹配。由于已知血小板中的P2 Y（12）受体调节粘附/活化和血栓生长/稳定性，从我们的结果我们可以通过类比推测，在少突胶质细胞中，P2 Y（12）受体信号传导可能有助于胶质过程向轴突的迁移和粘附以形成髓鞘。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
In the CNS, nucleotide receptors termed P2 receptors are identified on neurons and glial cells, mediating neuron-neuron, glia-glia and glia-neuron communication. In the present work, we qualify in vivo in the adult rat CNS the cellular/subcellular distribution of P2Y(12) receptor protein in cerebral cortex, white matter and subcortical nuclei (striatum and substantia nigra), by means of immunofluorescence-confocal, electron microscopy and Western blot analysis. P2Y(12) receptor immunoreactivity colocalizes neither with markers such as neuronal nuclei, neurofilament light chain, calbindin and tyrosine hydroxylase, nor with glial fibrillary acidic protein and isolectin B4, but with myelin basic protein and the oligodendrocyte marker RIP, in both cell bodies and processes, indicating therefore oligodendrocyte localization. Electron microscopy identifies P2Y(12) receptors in both the perikaryon and under the plasmalemma of oligodendrocyte cell bodies and radiating processes, until the paranodal region of fibers. By Western blot analysis, P2Y(12) receptor shows a specific band of 42-44 kDa, matching the molecular mass predicted from amino acid sequencing. Since in platelets P2Y(12) receptor is known to regulate adhesion/activation and thrombus growth/stability, from our results we could speculate by analogy that, in oligodendrocytes, P2Y(12) receptor signaling might contribute to the migration and adhesion of the glial processes to axons to be myelinated. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.