ANALYSIS OF THE NATURAL HUMAN-IGG ANTIBODY REPERTOIRE - LIFELONG STABILITY OF REACTIVITIES TOWARDS SELF-ANTIGENS CONTRASTS WITH AGE-DEPENDENT DIVERSIFICATION OF REACTIVITIES AGAINST BACTERIAL-ANTIGENS
ANALYSIS OF THE NATURAL HUMAN-IGG ANTIBODY REPERTOIRE - LIFELONG STABILITY OF REACTIVITIES TOWARDS SELF-ANTIGENS CONTRASTS WITH AGE-DEPENDENT DIVERSIFICATION OF REACTIVITIES AGAINST BACTERIAL-ANTIGENS
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DOI：
10.1002/eji.1830250929
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发表时间：
1995-09-01
影响因子：
5.4
通讯作者：
KAZATCHKINE, MD
中科院分区：
文献类型：
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作者：
LACROIXDESMAZES, S;MOUTHON, L;KAZATCHKINE, MD
我们采用定量免疫印迹技术分析了健康幼儿、青年男性和老年男性血清中IgG抗体反应性的特点。纯化的IgG与自身抗原的反应性的光密度模式在给定年龄组内和跨年龄组的个体之间高度保守。然而，在分析全血清中IgG的自身反应性时观察到个体间差异。一个显着的异质性在一个给定的年龄组内的个人和跨年龄组的特点是反应性的纯化IgG和IgG的全血清与细菌抗原。个体间差异在老年个体中比在其他年龄组的个体中更显著。IgG与细菌抗原的反应性的方差分析进一步表明，与年轻人和儿童相比，老年供体的库的多样性增加。我们的研究结果证明了整个生命过程中IgG自身反应库的稳定性，这与IgG抗体反应库随年龄增长而针对外来抗原的多样化形成对比。这些发现支持了这样一个概念，即自我反应抗体库在整个生命过程中被所有个体共享的一组有限的自身抗原积极选择。
We used a quantitative immunoblotting technique to analyze the repertoires of IgG antibody reactivities in the serum of healthy young children, young adult males and aged males with self and non-self antigens. Densitometric patterns of reactivity of purified IgG with self antigens were highly conserved between individuals within a given age group and across age groups. Inter-individual differences were observed, however, upon analysis of self reactivities of IgG in whole serum. A striking heterogeneity between individuals within a given age group and across age groups characterized the reactivity of purified IgG and of IgG in whole serum with bacterial antigens. Inter-individual differences were more marked among aged individuals than among individuals of other age groups. Analysis of variances of reactivities of IgG with bacterial antigens further demonstrated an increased diversity of repertoires of aged donors compared with those of young adults and children. Our results document the stability of the self-reactive repertoires of IgG throughout life, which contrasts with the diversification of the repertoire of IgG antibody reactivities directed toward foreign antigens with aging. These findings support the concept that self-reactive antibody repertoires are positively selected throughout life by a restricted set of self antigens shared by all individuals.