Development of radioimmunoassay for human leptin
Development of radioimmunoassay for human leptin
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DOI：
10.1006/bbrc.1996.0579
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发表时间：
1996-04-16
影响因子：
3.1
通讯作者：
Nakao, K
中科院分区：
文献类型：
--
作者：
Hosoda, K;Masuzaki, H;Nakao, K
利用重组人瘦素制备了抗人瘦素的抗血清，并建立了特异、灵敏的人瘦素放射免疫分析法。我们检测了瘦素样免疫反应（-LI）的培养液中的脂肪组织从皮下腹部脂肪。非肥胖受试者（17.6 <体重指数（BMI）< 23.0）的血浆人瘦素-LI浓度为5.6 +/- 1.3（平均值+/- SE）ng/ml，而肥胖受试者（29.0 < BMI）的血浆人瘦素-LI浓度为43.0 +/- 43.0 +/- 9.4（平均值+/- SE）ng/ml。在凝胶渗透色谱分析中，培养基和血浆中的瘦素-LI由重组人瘦素洗脱位置处出现的单一组分组成。这些发现表明，瘦素是从脂肪组织分泌到循环血液中作为一个大分子形式对应于重组瘦素。本研究建立的放射免疫分析方法为研究瘦素在人体内的生理和病理生理意义提供了有力的工具。(C)1996年学术出版社，Inc.
Using recombinant human leptin, we have produced an antiserum for human leptin and developed a radioimmunoassay (RIA) specific and sensitive for human leptin. We detected leptin-like immunoreactivity (-LI) in culture media of adipose tissue from subcutaneous abdominal fat in human. The plasma human leptin-LI concentration in nonobese subjects (17.6 < body mass index (BMI) < 23.0) was 5.6 +/- 1.3 (mean +/- SE) ng/ml, while that in obese subjects (29.0 < BMI) was 43.0 +/- 43.0 +/- 9.4 (mean +/- SE) ng/ml. In gel permeation chromatographic analyses, leptin-LI in culture media and plasma consisted of single component emerging at the elution position of recombinant human leptin. These findings indicate that leptin is secreted from the adipose tissue into the circulating blood as a large molecular form corresponding to recombinant leptin. The RIA developed in the present study will be a powerful tool to investigate the physiological and pathophysiological significance of leptin in human. (C) l996 Academic Press, Inc.