Protein recognition motifs of S gamma 3 DNA are statistically correlated with switch recombination breakpoints.
Protein recognition motifs of S gamma 3 DNA are statistically correlated with switch recombination breakpoints.
复制标题
S gamma 3 DNA 的蛋白质识别基序与开关重组断点具有统计相关性。
DOI：
10.1007/978-3-642-77633-5_18
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发表时间：
1992
影响因子：
--
通讯作者：
Kenter,AL
中科院分区：
文献类型：
--
作者：
Wuerffel,RA;Kenter,AL
免疫球蛋白 (Ig) 重 (H) 链类别开关允许表达成熟可变 (VDJ) 区和除 Cμ 之外的恒定 (C) 区（查看 [6]）。对经历同种型转换的 IgH 位点的分子分析发现，这一事件导致 Cμ 基因和插入基因组序列的删除 [18, 21]。开关重组的环出和删除模型预测开关 (S) 区域之间的插入 DNA 将被切除为一个圆圈 [9]。最近已从有丝分裂原刺激的脾细胞中分离出 Ig 开关重组的环状切除产物 [7,8,14,25]。这一发现证实了开关重组是通过环出和删除机制发生的。
The Immunoglobulin (Ig) heavy (H) chain class switch permits expression of a mature variable (VDJ) region with constant (C) regions other than Cμ (for review see [6]). Molecular analysis of IgH loci which have undergone isotype switching has led to the observation that this event results in the deletion of the Cμ gene and the intervening genomic sequences [18, 21]. The looping-out and deletion model for switch recombination predicts that the intervening DNA between switch (S) regions will be excised as a circle [9]. Circular excision products of Ig switch recombination have recently been isolated from mitogen stimulated spleen cells [7, 8, 14, 25]. This finding confirms that switch recombination occurs by the looping-out and deletion mechanism.