Crucial importance of length of fatty-acyl chains bound to the sn-2 position of phosphatidylglycerol for growth and photosynthesis of Synechocystis sp. PCC 6803
Crucial importance of length of fatty-acyl chains bound to the sn-2 position of phosphatidylglycerol for growth and photosynthesis of Synechocystis sp. PCC 6803
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与磷脂酰甘油 sn-2 位置结合的脂肪酰基链的长度对于集胞藻的生长和光合作用至关重要。
DOI：
10.1016/j.bbalip.2022.159158
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Hajime Wada
中科院分区：
文献类型：
--
作者：
Kaichiro Endoa;Masato Abe;Nobumasa Kawanishi;Haruhiko Jimbo;Koichi Kobayashi;Tomoko Suzuki;Noriko Nagata;Hideto Miyoshi;Hajime Wada
类囊体膜中的磷脂酰甘油（PG）对于光合生物的生长和光合作用至关重要。虽然类囊体膜中PG的n-2位只与C16脂肪酸酯化，但其n-2位C16脂肪酰基链的功能重要性尚未阐明。在本研究中，我们化学合成了不可代谢的PG分子：我们通过醚键分别在sn-1和sn-2位引入亚油酸（18：2，含有18个碳原子和2个双键的脂肪酸）和一种具有不同链长的饱和脂肪酸（12：0，14：0，16：0，18：0和20：0）。利用合成的醚连接的PG分子，我们检查了它们是否可以补充集胞藻的pgsA突变细胞的生长和光合作用。pgsA突变体不能合成PG，因此需要在培养基中添加外源PG来生长。突变体细胞的生长速率和光合活性取决于脂肪链的长度：结合16：0的PG分子种类最有效地补充突变体细胞的生长和光合作用，而脂肪链短于或长于16：0的其他PG分子种类的效果较差;特别是结合12：0的PG分子种类抑制突变体细胞的生长和光合活性。这些数据表明，结合到PG的thesn-2位的脂肪链的长度是至关重要的PG在生长和光合作用的性能。
Phosphatidylglycerol (PG) in thylakoid membrane is essential for growth and photosynthesis of photosynthetic organisms. Although thesn-2 position of PG in thylakoid membrane is exclusively esterified with C16fatty acids, the functional importance of the C16fatty-acyl chains at thesn-2 position has not been clarified. In this study, we chemically synthesized non-metabolizable PG molecules: we introduced linoleic acid (18:2, fatty acid containing 18 carbons with 2 double bonds) and one of the saturated fatty acids with different chain length (12:0, 14:0, 16:0, 18:0 and 20:0) by ether linkage to thesn-1 andsn-2 positions, respectively. With the synthesized ether-linked PG molecules, we checked whether they could complement the growth and photosynthesis ofpgsAmutant cells ofSynechocystissp. PCC 6803 to understand the importance of length of fatty chains at thesn-2 position of PG. ThepgsAmutant is incapable of synthesizing PG, so it requires exogenous PG added to medium for growth. The growth rate and photosynthetic activity of mutant cells depended on the length of fatty chains: the PG molecular species binding 16:0 most effectively complemented the growth and photosynthesis of mutant cells, and other PG molecular species with fatty chains shorter or longer than 16:0 were less effective; especially, those binding 12:0 inhibited the growth and photosynthetic activity of the mutant cells. These data demonstrate that length of fatty chains bound to thesn-2 position of PG is critical for PG performance in growth and photosynthesis.