Survey to detect tick‐borne encephalitis virus from human‐feeding ticks in Hokkaido, Japan
Survey to detect tick‐borne encephalitis virus from human‐feeding ticks in Hokkaido, Japan
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日本北海道从喂食人类的蜱中检测蜱传脑炎病毒的调查
DOI：
10.1111/1346-8138.15865
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Yoshii Kentaro
中科院分区：
文献类型：
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作者：
Sasaki Kenta;Honma Masaru;Nakao Minoru;Sasaki Mizuki;Hashimoto Yoshio;Ishida‐Yamamoto Akemi;Yoshii Kentaro
蜱虫感染是日本最北部岛屿北海道最常见的节肢动物相关皮肤病之一。蜱也是人类传染病的媒介。蜱传脑炎（TBE）是一种由TBE病毒（TBEV）引起的高度致命的中枢神经系统感染，最近在那里零星发生。然而，目前还没有关于人类蜱叮咬监测和TBEV在人类喂养蜱中流行的流行病学数据。本研究旨在澄清这些不确定的问题。2018年4月至8月期间，从北海道的150名门诊患者中收集了153只以人类为食的硬蜱。所有病例均未出现感染症状。对这些蜱进行形态学鉴定，并对幼仓鼠肾细胞进行细胞病变试验，以检测每只蜱中的TBEV。收集的蜱包括108只全沟硬蜱（1只若虫和107只成年雌性）、44只雌性卵形硬蜱和1只雌性日本血蜱。无蜱提取物显示细胞病变试验阳性结果，表明当前标本中不存在TBEV。然而，即使在北海道的北方和东部地区，被TBEV的可能传播媒介卵形硬蜱叮咬的人也在增加，因此从人食蜱中检测TBEV的调查对于监测TBE的发生仍然很重要。
A tick infestation is one of the most common arthropod‐related skin diseases in Hokkaido, the northernmost island of Japan. Ticks also act as an infectious disease vector for humans. Tick‐borne encephalitis (TBE), a highly mortal central nervous system infection caused by TBE virus (TBEV), has sporadically occurred there recently. However, there have been no epidemiological data on the current surveillance of human tick bites and the prevalence of TBEV in human‐feeding ticks. This study was performed to clarify those indeterminate issues. One hundred and fifty‐three ixodid ticks feeding on humans were collected from 150 outpatients in Hokkaido during the season of April to August 2018. None of the cases showed any infectious symptoms. These ticks were morphologically identified to species, and a cytopathic assay on baby hamster kidney cells was carried out to detect TBEV from each tick. The tick collection consisted of 108Ixodes persulcatus(one nymph and 107 adult females), 44 femaleIxodes ovatus, and one femaleHaemaphysalis japonica. No tick extracts showed positive results of the cytopathic assay, suggesting the non‐existence of TBEV in the present specimens. However, the survey to detect TBEV from human‐feeding ticks is still important to monitor the occurrence of TBE, because human tick bites byI. ovatus, a possible vector of TBEV, are increasing even in the northern and eastern areas of Hokkaido.