BETA-AMINOBUTYRIC ACID INDUCES THE ACCUMULATION OF PATHOGENESIS-RELATED PROTEINS IN TOMATO (LYCOPERSICON-ESCULENTUM L.) PLANTS AND RESISTANCE TO LATE BLIGHT INFECTION CAUSED BY PHYTOPHTHORA-INFESTANS
BETA-AMINOBUTYRIC ACID INDUCES THE ACCUMULATION OF PATHOGENESIS-RELATED PROTEINS IN TOMATO (LYCOPERSICON-ESCULENTUM L.) PLANTS AND RESISTANCE TO LATE BLIGHT INFECTION CAUSED BY PHYTOPHTHORA-INFESTANS
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DOI：
10.1104/pp.104.1.59
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发表时间：
1994-01-01
期刊：
影响因子：
7.4
通讯作者：
FLUHR, R
中科院分区：
文献类型：
--
作者：
COHEN, Y;NIDERMAN, T;FLUHR, R
番茄（Lycopersicon esculentum L.）用氨基丁酸异构体的水溶液喷洒植物，分析病程相关（PR）蛋白的积累或用晚疫病真菌病原致病疫霉（Phytophthorainfestans）攻击。氨基丁酸的β异构体诱导三种蛋白质的高水平积累：P14a、β-1，3葡聚糖酶和几丁质酶。这些蛋白质要么没有积累或积累到一个低得多的水平在α或γ-氨基丁酸处理的植物。用α-，β-和γ-氨基丁酸预处理的植物被保护长达11天的程度分别为35%，92%和6%，对致病疫霉的挑战感染。即使在施用化学品1时，β-氨基丁酸也能提供抗枯萎病的保护。d接种后。乙烯释放的检查表明，α-氨基丁酸诱导产生的乙烯水平比β-氨基丁酸高3倍，而γ-氨基丁酸不诱导乙烯产生。此外，银硫代硫酸盐，乙烯作用的有效抑制剂，并没有取消β-氨基丁酸诱导的抗性。结果是一致的可能性，β-氨基丁酸保护番茄叶片对晚疫病的机制，不介导的乙烯和PR蛋白可以参与诱导抗性。
Tomato (Lycopersicon esculentum L.) plants were sprayed with aqueous solutions of isomers of aminobutyric acid and were either analyzed for the accumulation of pathogenesis-related (PR) proteins or challenged with the late blight fungal agent Phytophthora infestans. The beta isomer of aminobutyric acid induced the accumulation of high levels of three proteins: P14a, beta-1,3 glucanase, and chitinase. These proteins either did not accumulate or accumulated to a much lower level in alpha- or gamma-aminobutyric acid-treated plants. Plants pretreated with alpha-, beta-, and gamma-aminobutyric acid were protected up to 11 d to an extent of 35, 92, and 6%, respectively, against a challenge infection with P. infestans. Protection by beta-aminobutyric acid was afforded against the blight even when the chemical was applied 1. d postinoculation. Examination of ethylene evolution showed that alpha-aminobutyric acid induced the production of 3-fold higher levels of ethylene compared with beta-aminobutyric acid, whereas gamma-aminobutyric acid induced no ethylene production. In addition, silver thiosulfate, a potent inhibitor of ethylene action, did not abolish the resistance induced by beta-aminobutyric acid. The results are consistent with the possibility that beta-aminobutyric acid protects tomato foliage against the late blight disease by a mechanism that is not mediated by ethylene and that PR proteins can be involved in induced resistance.