Structure of the mouse glial fibrillary acidic protein gene: implications for the evolution of the intermediate filament multigene family.
Structure of the mouse glial fibrillary acidic protein gene: implications for the evolution of the intermediate filament multigene family.
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DOI：
10.1093/nar/13.15.5527
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发表时间：
1985-08
影响因子：
14.9
通讯作者：
Joanna M. Balcarek;N. J. Cowan
中科院分区：
文献类型：
--
作者：
Joanna M. Balcarek;N. J. Cowan
我们报告了编码小鼠胶质纤维酸性蛋白（GFAP）的基因的完整序列，GFAP是星形胶质细胞特异性的中间丝（IF）蛋白。9.8 kb的基因包括9个外显子，内含子的大小从0.2到2.5 kb不等。GFAP基因与编码其他IF蛋白的基因的组织的比较揭示，IF基因的结构是高度保守的，尽管在氨基酸水平上存在相当大的分歧。因此，大多数的进化事件，导致插入子在IF基因的位置必须发生之前的复制和随后的分歧IF基因从一个假定的共同祖先序列。保守的基因组织与IF蛋白的结构特征无关。GFAP基因的一个奇怪的特征是在内含子中发现了大量的重复序列。存在六个重复的二核苷酸或三核苷酸束，加上两个不同的新序列的串联重复。一个重复是GFAP基因所独有的;另一个重复出现在小鼠基因组的其他地方，尽管频率相对较低。
We report the complete sequence of the gene encoding mouse glial fibrillary acidic protein (GFAP), the intermediate filament (IF) protein specific to astrocytes. The 9.8 kb gene includes nine exons separated by introns ranging in size from 0.2 to 2.5 kb. A comparison of the organization of the GFAP gene with that of genes encoding other IF proteins reveals that the structure of IF genes is highly conserved in spite of considerable divergence at the amino acid level. Thus, most of the evolutionary events leading to the placement of introns in IF genes must have occurred prior to the duplication and subsequent divergence of IF genes from a presumptive common ancestral sequence. The conserved gene organization is unrelated to structural features of IF proteins. A curious feature of the GFAP gene is the large number of repeated sequences found in the introns. Six tracts of reiterated di- or trinucleotides are present, plus tandem repeats of two different novel sequences. One repeat is unique to the GFAP gene; the other occurs elsewhere in the mouse genome, although at relatively low frequency.