The Rpf107 gene, a homolog of LOR, is required for the symbiotic nodulation of Robinia pseudoacacia
The Rpf107 gene, a homolog of LOR, is required for the symbiotic nodulation of Robinia pseudoacacia
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DOI：
10.1007/s00425-023-04280-3
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发表时间：
2024-01-01
期刊：
影响因子：
4.3
通讯作者：
Chou，Minxia
中科院分区：
文献类型：
--
作者：
Li，Yuanli;Wu，Yuanyuan;Chou，Minxia
主要结论Rpf 107参与根瘤菌的侵染过程和刺槐根瘤共生固氮的维持摘要LURP-one相关蛋白（LOR）家族在介导植物对生物和非生物胁迫的防御反应中起着关键作用。然而，我们对它在豆科植物和根瘤菌之间的共生相互作用中的功能的理解仍然有限。在这里，Rpf 107，LOR的同源物，被确定在刺槐（刺槐）。分析Rpf 107的亚细胞定位，并利用RNA干扰（RNAi）和过表达技术研究其功能。亚细胞定位分析表明，Rpf 107主要分布在质膜和细胞核中，沉默Rpf 107可抑制根瘤菌的侵染，抑制植株生长。Rpf 107-RNAi根瘤固氮区的感染细胞数也明显低于对照根瘤。值得注意的是，Rpf 107沉默导致类杆菌降解和结节的过早老化。相反，Rpf 107的过表达延缓了根瘤的衰老，延长了根瘤的固氮能力。这些结果表明Rpf 107参与了刺槐根瘤菌的侵染和共生固氮的维持。研究结果表明LOR蛋白家族的一个成员在豆科根瘤共生中发挥作用，这有助于阐明植物LOR蛋白家族的功能，全面了解豆科植物与根瘤菌共生的分子机制。
Main conclusionRpf107 is involved in the infection process of rhizobia and the maintenance of symbiotic nitrogen fixation in black locust root nodules.AbstractThe LURP-one related (LOR) protein family plays a pivotal role in mediating plant defense responses against both biotic and abiotic stresses. However, our understanding of its function in the symbiotic interaction between legumes and rhizobia remains limited. Here, Rpf107, a homolog of LOR, was identified inRobinia pseudoacacia(black locust). The subcellular localization of Rpf107 was analyzed, and its function was investigated using RNA interference (RNAi) and overexpression techniques. The subcellular localization assay revealed that Rpf107 was mainly distributed in the plasma membrane and nucleus.Rpf107silencing prevented rhizobial infection and hampered plant growth. The number of infected cells in the nitrogen fixation zone of theRpf107-RNAi nodules was also noticeably lower than that in the control nodules. Notably,Rpf107silencing resulted in bacteroid degradation and the premature aging of nodules. In contrast, the overexpression ofRpf107delayed the senescence of nodules and prolonged the nitrogen-fixing ability of nodules. These results demonstrate thatRpf107was involved in the infection of rhizobia and the maintenance of symbiotic nitrogen fixation in black locust root nodules. The findings reveal that a member of the LOR protein family plays a role in leguminous root nodule symbiosis, which is helpful to clarify the functions of plant LOR protein family and fully understand the molecular mechanisms underlying legume–rhizobium symbiosis.