Proteomic analysis of triclosan resistance in Salmonella enterica serovar Typhimurium.
Proteomic analysis of triclosan resistance in Salmonella enterica serovar Typhimurium.
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DOI：
10.1093/jac/dkn138
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发表时间：
2008-07
期刊：
影响因子：
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通讯作者：
M. Webber;N. Coldham;M. Woodward;L. Piddock
中科院分区：
文献类型：
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作者：
M. Webber;N. Coldham;M. Woodward;L. Piddock
目的 本研究的目的是测定和比较鼠伤寒沙门氏菌三种三氯生耐药突变体的蛋白质组，以鉴定与三氯生耐药相关的蛋白质。方法 使用二维液相色谱质量分离测定三种不同但同基因的三氯生抗性突变体的蛋白质组。然后使用生物信息学来鉴定和定量胰蛋白酶肽以确定蛋白质表达。结果三氯生抗性突变体的蛋白质组学分析鉴定了一组参与丙酮酸或脂肪酸生产的常见蛋白质，在所有突变体中具有差异表达，但也证明了与每种表型相关的特定表达模式。结论 这些数据表明，沙门氏菌可以通过不同的途径发生三氯生耐药性，并证明了一种新的三氯生耐药性网络，该网络可能与其他接触三氯生的病原菌有关，并可能为抗菌药物的开发提供新的靶点。
OBJECTIVES The aim of this study was to determine and compare the proteomes of three triclosan-resistant mutants of Salmonella enterica serovar Typhimurium in order to identify proteins involved in triclosan resistance. METHODS The proteomes of three distinct but isogenic triclosan-resistant mutants were determined using two-dimensional liquid chromatography mass separation. Bioinformatics was then used to identify and quantify tryptic peptides in order to determine protein expression. RESULTS Proteomic analysis of the triclosan-resistant mutants identified a common set of proteins involved in production of pyruvate or fatty acid with differential expression in all mutants, but also demonstrated specific patterns of expression associated with each phenotype. CONCLUSIONS These data show that triclosan resistance can occur via distinct pathways in Salmonella, and demonstrate a novel triclosan resistance network that is likely to have relevance to other pathogenic bacteria subject to triclosan exposure and may provide new targets for development of antimicrobial agents.