Angiopellosis as an Alternative Mechanism of Cell Extravasation.
Angiopellosis as an Alternative Mechanism of Cell Extravasation.
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血管扩张症作为细胞外渗的替代机制
DOI：
10.1002/stem.2451
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发表时间：
2017-01
期刊：
影响因子：
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通讯作者：
Cheng K
中科院分区：
文献类型：
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作者：
Allen TA;Gracieux D;Talib M;Tokarz DA;Hensley MT;Cores J;Vandergriff A;Tang J;de Andrade JB;Dinh PU;Yoder JA;Cheng K
当静脉注射时，干细胞具有回家和旅行到受损组织的能力。为了使细胞发挥其治疗作用，它们必须穿过血管壁并进入周围组织。外渗注射干细胞用于退出的机制尚未被表征。使用活体显微镜和具有表达GFP的脉管系统的转基因斑马鱼品系Tg（fli 1a：egpf），我们记录了注射的干细胞与白色血细胞（WBC）相比的体内详细外渗过程。虽然白细胞通过标准的渗出过程离开血管，但注射的心脏和间充质干细胞经历了与渗出明显不同的独特外渗方法。在这里，血管壁经历了广泛的重塑，以允许细胞离开管腔，而注射的细胞在活动中保持明显的被动。我们将这一过程称为血管扩张，它代表了细胞外渗的替代机制，以取代流行的渗出理论。干细胞2017;35：170-180
Stem cells possess the ability to home in and travel to damaged tissue when injected intravenously. For the cells to exert their therapeutic effect, they must cross the blood vessel wall and enter the surrounding tissues. The mechanism of extravasation injected stem cells employ for exit has yet to be characterized. Using intravital microscopy and a transgenic zebrafish line Tg(fli1a:egpf) with GFP‐expressing vasculature, we documented the detailed extravasation processes in vivo for injected stem cells in comparison to white blood cells (WBCs). While WBCs left the blood vessels by the standard diapedesis process, injected cardiac and mesenchymal stem cells underwent a distinct method of extravasation that was markedly different from diapedesis. Here, the vascular wall undergoes an extensive remodeling to allow the cell to exit the lumen, while the injected cell remains distinctively passive in activity. We termed this process Angio‐pello‐sis, which represents an alternative mechanism of cell extravasation to the prevailing theory of diapedesis. Stem Cells 2017;35:170–180