Mechanisms of IFN-gamma induction by natural killer cell stimulatory factor (NKSF/IL-12). Role of transcription and mRNA stability in the synergistic interaction between NKSF and IL-2.
Mechanisms of IFN-gamma induction by natural killer cell stimulatory factor (NKSF/IL-12). Role of transcription and mRNA stability in the synergistic interaction between NKSF and IL-2.
复制标题
DOI：
10.4049/jimmunol.148.1.92
复制
发表时间：
1992-01
影响因子：
4.4
通讯作者：
S. Chan;Michiko Kobayashi;D. Santoli;B. Perussia;G. Trinchieri
中科院分区：
文献类型：
--
作者：
S. Chan;Michiko Kobayashi;D. Santoli;B. Perussia;G. Trinchieri
我们研究了NK细胞刺激因子(NKSF/IL-12)刺激的人T淋巴细胞产生干扰素-γ的分子机制。我们发现NKSF与IL-2和佛波酯有协同作用，可在PHA激活的T细胞中诱导干扰素-γ的mRNA积聚。NKSF在转录和转录后水平调节PHA母细胞和T白血病细胞系TALL-103/2中干扰素-γ的mRNA表达。根据核连续分析，NKSF增加了这两种类型细胞中干扰素-伽马的转录速率。然而，NKSF和IL-2之间的协同作用只能在mRNA稳定的水平上得到证实，而且这两种细胞因子都需要增加干扰素-γ的半衰期。
We have investigated the molecular mechanisms regulating IFN-gamma production in human T lymphocytes stimulated by NK cell stimulatory factor (NKSF/IL-12). We show that NKSF synergizes with IL-2 and phorbol diesters inducing the accumulation of IFN-gamma mRNA in PHA-activated T cell blasts. NKSF regulates IFN-gamma mRNA expression in PHA blasts and the T leukemia cell line, TALL-103/2, at both the transcriptional and posttranscriptional levels. NKSF increases the transcriptional rate for IFN-gamma in both these cell types, as determined by nuclear run-on analysis. However, synergy between NKSF and IL-2 can be demonstrated only at the level of mRNA stability, and both cytokines are required to increase IFN-gamma mRNA half-life.